We narrowed to 13,278 results for: cDNA

1. Carl-YeNL-KDEL_pcDNA3

   Plasmid

   #89516

   Purpose

   Yellow color variant of bright luminescent protein, targeted to the endoplasmic reticulum

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   YeNL
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO C1-APEX2

   Plasmid

   #129641

   Purpose

   Express ER-localized C1-APEX2 fusion in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   C1(1-29)-APEX2-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Myc-Smad8

   Plasmid

   #11745

   Depositor

   Jeff Wrana

   Article

   Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

   Insert

   SMAD8 (Smad9 Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pcDNA3-FLAG-Fos DD

   Plasmid

   #8968

   Depositor

   John Blenis

   Article

   Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

   Insert

   c-Fos (Fos Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S362D, S374D

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5-FRT-TO-Nsp8

   Plasmid

   #157694

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3-mRuby2-MACROD1_d77

   Plasmid

   #172588

   Purpose

   For transient expression of C-terminal mRuby2-tagged MACROD1del1-77

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD1 (MACROD1 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-76

   Promoter

   CMV

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Ha-Brca1 S1387A

   Plasmid

   #32532

   Depositor

   Michael Kastan

   Article

   Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

   Insert

   Brca1 (BRCA1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S1387A

   Promoter

   CMV/Rabbit B-Globin

   Available since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-LYRa11-RBD-8h

   Plasmid

   #161822

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T324-D522)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)-FluoSTEP-JNKAR

   Plasmid

   #181857

   Purpose

   Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-JNKAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid

    #181856

    Purpose

    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AktAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT/TO-V5-SHLD3

    Plasmid

    #114124

    Purpose

    Expresses N-terminally tagged V5-SHLD3 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD3 (SHLD3 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Aug. 23, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Carl-CeNL-KDEL_pcDNA3

    Plasmid

    #89509

    Purpose

    Cyan color variant of bright luminescent protein, targeted to the endoplasmic reticulum

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+) HIPK3 Sense

    Plasmid

    #60634

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Homeodomain-interacting protein kinase 3 (HIPK3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pY018 (pcDNA3.1-hPdCpf1)

    Plasmid

    #69990

    Purpose

    Expresses humanized PdCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPdCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.2-V5-DEST-hSFRP1-VF

    Plasmid

    #189542

    Purpose

    Protein expression of SFRP1 in mammalian cells.

    Depositor

    Haian Fu

    Insert

    secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid

    #92305

    Purpose

    Expression of humanized Mb3Cpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huMb3Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO DNAJB3

    Plasmid

    #19471

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB3 (Dnajb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-SARS-1-RBD-8h

    Plasmid

    #161821

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1+_V5-ANKRD52

    Plasmid

    #92012

    Purpose

    Expresses V5-ANKRD52

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    ANKRD52 (ANKRD52 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-FRT-TO-FH-Nsp12

    Plasmid

    #157710

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp12 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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