We narrowed to 5,091 results for: codon optimized

1. pLenti6.3-SaCas9-BlastR

   Plasmid

   #220966

   Purpose

   Expresses human codon-optimized SaCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-T3-CjCAS9-pA

   Plasmid

   #131543

   Purpose

   Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   FLAG-SV40NLS, SV40NLS, and polyA

   Expression

   Mammalian

   Mutation

   Human codon-optimized

   Promoter

   CAG, T3

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. APLF-c001

   Plasmid

   #173077

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   APLF (APLF Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K360-V448, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSL2783 (pTnsC-VP64_Vch)

   Plasmid

   #200896

   Purpose

   Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchTnsC-VP64
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVRa41_491

   Plasmid

   #49702

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF41_491
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDNR-SpCas9

   Plasmid

   #80425

   Purpose

   Used for in vitro transcription of SpCas9 mRNA.

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   none

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMXS-IRES-Blast coUGP2

   Plasmid

   #211531

   Purpose

   Expresses codon optimized UGP2

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   UGP2 (UGP2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

   Plasmid

   #47550

   Purpose

   Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   ttTi5605 sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKMD106e - intein-Cas9(S219)

   Plasmid

   #64190

   Purpose

   Expresses intein-Cas9(S219) in mammalian cells

   Depositor

   David Liu

   Article

   Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

   Insert

   mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
   

   Use

   CRISPR

   Tags

   NLS - 3x FLAG (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p-mCherry-C1-GtACR1

    Plasmid

    #85464

    Purpose

    Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

    Insert

    Synthetic construct ACR1 gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCHA1.1-MFSD12-V5

    Plasmid

    #149508

    Purpose

    Stable expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti6.3-SauriCas9-BlastR

    Plasmid

    #220967

    Purpose

    Expresses human codon-optimized SauriCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SauriCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p-mCherry-C1-GtACR2

    Plasmid

    #85463

    Purpose

    Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al J Biol Chem. 2016 Feb 19;291(8):4121-7.

    Insert

    Synthetic construct ACR2 gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSmart-EF1a-Nla-cas5

    Plasmid

    #178878

    Purpose

    Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas5c-bGH polyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSmart-EF1a-Nla-cas8

    Plasmid

    #178880

    Purpose

    Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas8c-bGH PolyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSmart-EF1a-Nla-cas11

    Plasmid

    #178882

    Purpose

    Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas11c-bGH polyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSmart-EF1a-Nla-cas7

    Plasmid

    #178879

    Purpose

    Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas7c-bGH PolyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ARH1-c003

    Plasmid

    #173093

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRH (ADPRH Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E2-L357, codon optimized

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSL2669 (pTnsABf_Vch)

    Plasmid

    #200897

    Purpose

    Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsA-TnsB
    

    Use

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ALC1-c005

    Plasmid

    #173092

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge