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We narrowed to 25,709 results for: nes

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Showing: 2721 - 2740 of 25709 results

  3. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  4. G protein alpha-s-mCherry

    Plasmid
    #66968
    Purpose
    G protein alpha-s internally tagged with mCherry and EE epitope
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha- s…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66968-plasmid-map-sequence-id-121428

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  5. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid
    #135766
    Purpose
    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.
    Depositor
    Nadia Mercader Huber
    Article
    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6
    Insert
    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    Use
    Zebrafish
    Promoter
    UAS
    Available Since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    135766-plasmid-map-sequence-id-270202

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  7. G protein alpha-s-mCerulean

    Plasmid
    #66084
    Purpose
    G protein alpha-s internally tagged with mCerulean and EE epitope
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCerulean (Gnas Aequorea victoria, Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available Since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    66084-plasmid-map-sequence-id-118926

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  10. PGL4.11-COL1A1double mutants

    Plasmid
    #230918
    Purpose
    Double mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.
    Depositor
    Avi Rosenberg
    Article
    Yang et al Lab Invest. 2024 May;104(5):100336. doi:
    Insert
    Human CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant SMAD and TEAD binding…
    Promoter
    COL1A1 with mutant SMAD and TEAD elements
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230918-plasmid-map-sequence-id-460669

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  15. pαH-S-GSAS/XBB.1.5

    Plasmid
    #212991
    Purpose
    "Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 variant
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-GSAS/XBB.1.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    (T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q,Q183E…
    Promoter
    CMV
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212991-plasmid-map-sequence-id-422311

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Showing: 2721 - 2740 of 25709 results