We narrowed to 13,220 results for: CRISPR-Cas9

1. pRha-ABE8e-SpCas9-NG

   Plasmid

   #201190

   Purpose

   Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

   Depositor

   Jonathan Yen

   Article

   Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

   Insert

   ABE8e-SpCas9-NG
   

   Use

   Tags

   8xHIS and BP-NLS

   Expression

   Bacterial

   Mutation

   Promoter

   pRhaBAD

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

   Plasmid

   #169097

   Purpose

   Cas9 and Citrine expression in transfected cells

   Depositor

   Marianne Bronner

   Article

   Gandhi et al Development. 2021 Mar 9. pii: dev.193565

   Insert

   Cas9-2A-Citrine
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA47_dCas9-GFP_NOT_iC

   Plasmid

   #231418

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVA47_dCas9-GFP_NOT_iA

   Plasmid

   #231417

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

   Plasmid

   #234737

   Purpose

   All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

   Depositor

   Kirill Martemyanov

   Article

   Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

   Insert

   Nrl (Nrl Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2 NT sgRNA3

   Plasmid

   #164929

   Purpose

   Expresses Non-targeting sgRNA3 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA3 (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2 NT sgRNA5

   Plasmid

   #164930

   Purpose

   Expresses Non-targeting sgRNA5 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA5 (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

   Plasmid

   #197500

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-Cas9-VQR-Blast

   Plasmid

   #87155

   Purpose

   lentiviral expression of SpCas9-VQR (NGA PAM restricted)

   Depositor

   Daniel Bauer

   Article

   Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

   Insert

   SpCas9-VQR(D1135V,R1335Q,T1337R)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D1135V,R1335Q,T1337R

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-sgAMPKa2-Cas9-GFP

    Plasmid

    #208050

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2

    Depositor

    Naama Kanarek

    Article

    Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

    Insert

    sgPRKAA2 (PRKAA2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

    Plasmid

    #234826

    Purpose

    STITCHR R2Tocc editor

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    nCas9h840a-xten-R2Tocc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

    Plasmid

    #138418

    Purpose

    CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BioTAP-tag

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1

    Plasmid

    #138419

    Purpose

    CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BioTAP-tag

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

    Plasmid

    #194283

    Purpose

    EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

    Plasmid

    #121824

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9/LSD1 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

    Plasmid

    #234883

    Purpose

    non-targeting CRISPRi control

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

    Plasmid

    #192133

    Purpose

    Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9-KY
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Hsp1-Hsp2Cas9 (K390A, Y446A)

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

    Plasmid

    #121825

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9/p300core (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR

    Plasmid

    #121822

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9/VPR (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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