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pcDNA-Nup37-EGFP-polyA

Plasmid

#104562

Purpose

Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Nup37 (Nup37 Mouse)

Tags

EGFP tag

Expression

Mammalian

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-K40R

Plasmid

#64059

Purpose

EGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pBS-Isce1-eno2:egfp

Plasmid

#61394

Purpose

Expresses GFP in neurons of transgenic zebrafish

Depositor

Edward Burton

Article

Bai et al Nucleic Acids Res. 2007;35(19):6501-16.

Insert

eno2 regulatory element

Use

Zebrafish expression

Promoter

eno2

Available Since

Dec. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-p53 tethered

Plasmid

#49245

Purpose

expresses two human WT p53s tethered together and also expresses EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53-p53 (TP53 Human)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP7 (102-220)

Plasmid

#42314

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Δ1-102

Promoter

CMV

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
pSMALB-ATF4.14

Plasmid

#155034

Purpose

Fluorescent reporter for ATF4 translation, Positive Control

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 and uORF2 start codons mutated

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-M2-S2-S3-S4T3D

Plasmid

#33302

Depositor

Terence Dermody

Article

Kobayashi et al Virology. 2010 Mar 15;398(2):194-200. Ep

Inserts

S2 (S2 Orthoreovirus T3D)
S3 (S3 Orthoreovirus T3D)
S4 (S4 Orthoreovirus T3D)
M2 (M2 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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hiActTS-GR

Plasmid

#79775

Purpose

high FRET control for ActTS-GR

Depositor

Tetsuya Kitaguchi

Article

Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

Insert

hiActTS-GR

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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CRT-GFP pcDNA3.1

Plasmid

#80978

Purpose

mammalian expression of CRT-GFP

Depositor

Peter Steyger

Article

Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

Insert

CRT (Calr Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

no KDEL

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3 Connexin43-GFP-APEX

Plasmid

#44440

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

Connexin43 -EGFP - pea APEX

Expression

Mammalian

Mutation

K14D, W41F, E112K mutations on APEX

Promoter

CMV

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti.pFOXA2.GFP

Plasmid

#59404

Purpose

expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

Depositor

Kai-Christian Sonntag

Article

Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

Insert

400 bp FOXA2 promoter, eGFP (FOXA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pFOXA2

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX_DSB_Spectrum-V2

Plasmid

#192475

Purpose

Lentiviral DSB Spectrum V2 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP3fast

Plasmid

#74422

Purpose

Express fluorescent calcium indicator GCaMP3fast in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

Insert

GCaMP3fast

Expression

Mammalian

Mutation

W4Y D356A

Promoter

CMV

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-LAP tag

Plasmid

#67618

Purpose

A pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-termnal LAP (eGFP-TEV-S)

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pHJ321

Plasmid

#20676

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Thr 558 to Alanine

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.3b

Plasmid

#111056

Purpose

Expresses dLight1.3b in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.3b

Tags

Flag tag

Expression

Mammalian

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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ITPKA_3xmNeonGreen

Plasmid

#129606

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

F-tractin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1neo-NLucYAP15

Plasmid

#107610

Purpose

measure LATS activity by Luciferase Assay

Depositor

Xiaolong Yang

Article

Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

Insert

NLucYAP15

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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EHD2-mCherry

Plasmid

#45933

Purpose

caveolar protein, confines caveolae to the plasma membrane

Depositor

Ari Helenius

Article

Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

Insert

Eps-15 homology domain-containing protein2 (EHD2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC)

Plasmid Kit

#1000000175

Purpose

Plasmids and Gateway entry vectors for fluorescent sensors of diverse physiological parameters in cells.

Depositor

Adam Cohen

Application

Gene Expression and Labeling

Vector Type

Lentiviral, Mammalian Expression

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only