Addgene

We narrowed to 878 results for: RK2

Showing: 521 - 560 of 878 results

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pGEX-parkin W403A-C431S

Plasmid

#45977

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin W403A-C431S (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Doub…

Promoter

tac

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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V5-14-3-3-e

Plasmid

#229426

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

YWHAE (YWHAE Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MOB1B

Plasmid

#229428

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MOB1B (MOB1B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBI-BFP-NL1

Plasmid

#206153

Purpose

pBI-BFP-NL1

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

Neuroligin-1 (Nlgn1 Mouse)

Use

Tags

Bicistronic BFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-Rab12 Q101L

Plasmid

#207347

Purpose

Bacterial expression of Rab12

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

Rab12 (RAB12 Human)

Use

Tags

2xHis

Expression

Bacterial

Mutation

Q101L

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pmcb306-GFP-Rab12-mito

Plasmid

#207349

Purpose

Mammalian expression of Rab12-mito

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

Rab12-mito (RAB12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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CMV EGFP-PPM1H-H153D

Plasmid

#200432

Purpose

Mammalian expression of H153D mutation in PPM1H

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H

Use

Tags

EGFP

Expression

Mammalian

Mutation

H153D

Promoter

CMV

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mYwhae -2

Plasmid

#198498

Purpose

lentiviral stable expression of mYwhae gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mYwhae (Ywhae Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mYwhae - 1

Plasmid

#198497

Purpose

lentiviral stable expression of mYwhae gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mYwhae (Ywhae Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pPPC018.AAV

Plasmid

#171146

Purpose

Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

hAAVS1 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC019.AAV

Plasmid

#171147

Purpose

Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

hAAVS1 scRNA
J1-BBa_J23117-mRFP

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J1-BBa_J23117

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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HA-TEAD1

Plasmid

#229411

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

TEAD1 (TEAD1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBMN-mCherry-Parkin

Plasmid

#59419

Purpose

to make mCherry-Parkin stable cell line with retrovirus

Depositor

Richard Youle

Article

Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

Insert

mCherry-Parkin (PRKN Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK1

Plasmid

#199643

Purpose

Expresses VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN-YFP-Parkin

Plasmid

#59416

Purpose

to make YFP-Parkin stable cell line with retrovirus

Depositor

Richard Youle

Article

Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

Insert

YFP-Parkin (PRKN Human)

Use

Retroviral

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Parkin

Plasmid

#213547

Purpose

Transient mammalian expresssion of untagged Parkin

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-parkin C431S

Plasmid

#45877

Depositor

Edward Fon

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C431S mutation (ubiquitin acceptor site)

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
Myc-RILPL1

Plasmid

#201470

Purpose

For overexpression in cultured cells to visualize RILPL1 protein interactions.

Depositor

Suzanne Pfeffer

Article

Pal et al Sci Adv. 2023 Dec 15;9(50):eadj1205. doi

Insert

RILPL1 (RILPL1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Parkin

Plasmid

#187897

Purpose

Mammalian cell expression of untagged Parkin

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

PRKN (PRKN Human)

Use

Tags

None

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP-pk-S65A

Plasmid

#115619

Purpose

Expresses human Parkin S65A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S65A

Promoter

CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM4941 (pCV0053)

Plasmid

#120083

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-parkin W403A

Plasmid

#45876

Depositor

Edward Fon

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

W403A mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
GFP-STAM1

Plasmid

#164628

Purpose

Expresses STAM1 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

STAM1 (STAM Human)

Use

Tags

cycle 3 GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM4951 (pCV0063)

Plasmid

#120084

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
KCNJ6_pCSdest

Plasmid

#53804

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

KCNJ6 (KCNJ6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
sgCTRL-hygro

Plasmid

#199639

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP-pk-H302A

Plasmid

#115618

Purpose

Expresses human Parkin H302A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

H302A

Promoter

CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP-pk-F146A

Plasmid

#115616

Purpose

Expresses human Parkin F146A in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

F146A

Promoter

CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP-pk-N273K

Plasmid

#115617

Purpose

Expresses human Parkin N273K in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

N273K

Promoter

CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-parkin W403A

Plasmid

#227357

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin W403A (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-parkin L107K

Plasmid

#227363

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin L107K (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MKI-P2A-GFP-DOX-N

Plasmid

#229407

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MKI-P2A-GFP

Plasmid

#229405

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MOB1A

Plasmid

#229427

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MOB1A (MOB1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-parkin R104A

Plasmid

#227358

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin R104A (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-DARPin-E11

Plasmid

#226784

Purpose

Expression of His-tagged DARPin E11 in E. coli

Depositor

Sebastian Mathea

Article

Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

Insert

DARPin E11

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-parkin Ubl P37L

Plasmid

#227366

Purpose

For bacterial expression of mutated parkin Ubl.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin 1-76 P37L (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-parkin D106A

Plasmid

#227362

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin D106A (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-parkin V105K

Plasmid

#227361

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin V105K (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-parkin T103A

Plasmid

#227360

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin T103A (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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