We narrowed to 682 results for: RPL

1. P3-Lenti-dCas9-Tet1-GFP

   Plasmid

   #190729

   Purpose

   Lentiviral construct to express spdCas9-Tet1-GFP.

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   SV40 NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   UbC

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GFP Cav2.2 human -HAgHA-minus exon 18a -Short-Cterm - Arg -in pcDNA3

   Plasmid

   #198916

   Purpose

   N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector

   Depositor

   Annette Dolphin

   Article

   Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

   Insert

   Cav2.2 (CACNA1B Human)
   

   Use

   Tags

   N-terminal GFP, internal double HA

   Expression

   Mammalian

   Mutation

   exon 18a deleted, exon 19 starts 3 nucleotides ea…

   Promoter

   CMV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. P3-dCas9-Tet1-GFP-Puro

   Plasmid

   #190728

   Purpose

   Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   3XFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   pCAG

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMO14

   Plasmid

   #235736

   Purpose

   Expression of human VPS34 complex II in mammalian cells

   Depositor

   Roger Williams

   Article

   Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi:

   Inserts

   ATG14L (ATG14 Human)
   UVRAG (UVRAG Human)
   EGFP
   BECN1 (BECN1 Human)
   PIK3C3 (PIK3C3 Human)
   PIK3R4 (PIK3R4 Human)
   

   Use

   Tags

   2xStrep-tagII-2xTEV-ZZ and ZZ-3xTEV

   Expression

   Mammalian

   Mutation

   N256S

   Promoter

   CMV

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pILGFP1DFB

   Plasmid

   #185871

   Purpose

   A parental plasmid for in vivo gene amplification at RPL25 locus

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pILGFP3AA5

   Plasmid

   #185875

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pILGFP3AG4

   Plasmid

   #185874

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pILGFP3AE4

   Plasmid

   #185873

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pILGFP3A5C

   Plasmid

   #185872

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFP3AG4ARSd

    Plasmid

    #185876

    Purpose

    A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pILAC3

    Plasmid

    #185890

    Purpose

    In vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 F96C; Xd.CRtYB E83K

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pIT6EG7ml

    Plasmid

    #185886

    Purpose

    In vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (14 copy).

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 F96W N127W

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pIT6EG7mlh

    Plasmid

    #185887

    Purpose

    In vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (23 copy).

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 F96W N127W

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pILAC2

    Plasmid

    #185889

    Purpose

    In vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    see comments
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 F96C; Xd.CRtYB E83K

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIT6EG7m

    Plasmid

    #185885

    Purpose

    A backbone plasmid for in vivo gene amplification (HamAmp) of a monoterpene synthetic module (+ GPP synthase without a monoterpene synthase) at RPL25 locus (14 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mEmerald-UbiquitinC-C-18

    Plasmid

    #54294

    Purpose

    Localization: Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. mEmerald-UbiquitinC-N-18

    Plasmid

    #54295

    Purpose

    Localization: Membrane , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. mVenus-UbiquitinC-N-18

    Plasmid

    #56339

    Purpose

    Localization: Membrane , Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. mCherry-UbiquitinC-C-18

    Plasmid

    #55149

    Purpose

    Localization: Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

20. mVenus-UbiquitinC-C-18

    Plasmid

    #56393

    Purpose

    Localization: Membrane, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

21. mCherry-UbiquitinC-N-18

    Plasmid

    #55150

    Purpose

    Localization: Membrane , Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    UbiquitinC (UBA52 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 2-76 of NP_003324.1

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pGC157

    Plasmid

    #19682

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28
    

    Use

    Entry clone

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-IV-3xFLAG/HA/NBL10

    Plasmid

    #110568

    Purpose

    Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag

    Depositor

    Jeffrey Friedman, Alexander Nectow

    Insert

    3xFLAG/HA-tagged anti-GFP VHH
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGC114

    Plasmid

    #19622

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<1xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Rp51a_SUS1 5'SS

    Plasmid

    #100991

    Purpose

    Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GGTAtGT->tGTAtGa 5'SS mutant

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Rp51a_No_BP-pUC18

    Plasmid

    #100992

    Purpose

    Contains model Rp51a with a TACTAAC->GTTAGTG BP mutataion

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    TACTAAC->GTTAGTG BP mutataion

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLG-PLint

    Plasmid

    #33145

    Depositor

    Michael Rosbash

    Article

    Legrain et al Cell. 1989 May 19;57(4):573-83.

    Insert

    RP51A-synthetic minimal intron (RPS17A Budding Yeast)
    

    Use

    Tags

    LacZ

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pG1AK-mCherry

    Plasmid

    #71737

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Insert

    mCherry
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRplsWT

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YIplac204-pOst1-ESCargo(Crimson)

    Plasmid

    #140154

    Purpose

    Expresses a vacuolar cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

    Insert

    pOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRRP51A+intron-LacZ (pHZ18)

    Plasmid

    #33131

    Depositor

    Michael Rosbash

    Article

    Teem et al Proc Natl Acad Sci U S A. 1983 Jul;80(14

    Insert

    RP51A+intron-LacZ (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pGST2-Ub CPS (8-16)

    Plasmid

    #17631

    Depositor

    James Hurley

    Article

    Lee et al Nat Struct Mol Biol. 2006 Mar . 13(3):26

    Insert

    Ub CPS (8-16) (RPL40A Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

32. pG1AK-sfGFP

    Plasmid

    #71739

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRplsWT

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGC185

    Plasmid

    #19624

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pG1AK-PheB

    Plasmid

    #71738

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Insert

    PheB
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRplsWT

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGC91

    Plasmid

    #19621

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Rp51a Hyperstabilized 5'SS

    Plasmid

    #100988

    Purpose

    pUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGT

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    5'ss mut ATG/GTATGT -> AAG/GTAAGT

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. RP51A-NoBS-NoPseudoBS

    Plasmid

    #100986

    Purpose

    RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLG-Acc

    Plasmid

    #33148

    Depositor

    Michael Rosbash

    Article

    Legrain et al Cell. 1989 May 19;57(4):573-83.

    Insert

    RP51A-synthetic minimal intron (RPS17A Budding Yeast)
    

    Use

    Tags

    LacZ

    Expression

    Yeast

    Mutation

    AccI site in intron was cut/filled/ligated to cha…

    Promoter

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pLG-Sty

    Plasmid

    #33149

    Depositor

    Michael Rosbash

    Article

    Legrain et al Cell. 1989 May 19;57(4):573-83.

    Insert

    RP51A-synthetic minimal intron (RPS17A Budding Yeast)
    

    Use

    Tags

    LacZ

    Expression

    Yeast

    Mutation

    Sty1 site upstream of intron was cut/filled/ligat…

    Promoter

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pG4

    Plasmid

    #162605

    Purpose

    Edit Ade2 gene in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge