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Showing: 651 - 700 of 4942 results

  1. pUCP20 PilB113*Q119A

    Plasmid
    #162867
    Purpose
    Expression of PilB Amber with a C-terminal histag
    Depositor
    Katrina Forest
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    Q113Amber, Q119A
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162867-plasmid-map-sequence-id-329259

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  2. pUCP20 PilB113*L197D

    Plasmid
    #162868
    Purpose
    Expression of PilB Amber with a C-terminal histag
    Depositor
    Katrina Forest
    Insert
    PilB
    Use
    Tags
    Histidine tag (H6x)
    Expression
    Bacterial
    Mutation
    Q113Amber, L193D
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162868-plasmid-map-sequence-id-329261

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  5. Rp51a_10bp 5'SS_No BP or pBP_pUC18

    Plasmid
    #100989
    Purpose
    Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
    Depositor
    Aaron Hoskins
    Article
    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1
    Insert
    RP51A (RPS17A Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    100989-plasmid-map-sequence-id-194327

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  6. Rp51a_SUS1 5'ss_No_BP_pBP-pUC18

    Plasmid
    #100990
    Purpose
    Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
    Depositor
    Aaron Hoskins
    Article
    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1
    Insert
    RP51A (RPS17A Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    100990-plasmid-map-sequence-id-194330

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  8. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid
    #181748
    Purpose
    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Type
    Empty backbone
    Use
    Substrate plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181748-plasmid-map-sequence-id-354677

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  9. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

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  18. pUC19-hsg(G:H)

    Plasmid
    #138259
    Purpose
    Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Insert
    mCherry targeting sgRNAs G and H
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    138259-plasmid-map-sequence-id-273638

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  38. pUCmini-iCAP-AAV.CAP-B10

    Plasmid
    #175004
    Purpose
    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175004-plasmid-map-sequence-id-347131

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  45. H52-pUCM-hNGN2-inv

    Plasmid
    #110492
    Purpose
    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm
    Depositor
    Michael Ward
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    Use
    CRISPR, Cre/Lox, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, EF-1 alpha, and TRE3G
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110492-plasmid-map-sequence-id-207872

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  49. pUCmini-iCAP-AAV.CAP-B22

    Plasmid
    #175005
    Purpose
    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175005-plasmid-map-sequence-id-347133

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Showing: 651 - 700 of 4942 results