We narrowed to 24,805 results for: 034

1. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX

   Plasmid

   #214284

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX

   Plasmid

   #214285

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX

   Plasmid

   #214286

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX

   Plasmid

   #214287

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Dynein-2_complex

   Plasmid

   #132536

   Purpose

   Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Inserts

   DYNC2H1 (DYNC2H1 Human)
   DYNC2LI1 (DYNC2LI1 Human)
   WDR34 (WDR34 Human)
   WDR60 (WDR60 Human)
   DYNLL1 (DYNLL1 Human)
   DYNLL2 (DYNLL2 Human)
   DYNLRB1 (DYNLRB1 )
   DYNLRB2 (DYNLRB2 Human)
   DYNLT1 (DYNLT1 Human)
   TCTEX1D2 (TCTEX1D2 Human)
   DYNLT3 (DYNLT3 Human)
   LC8-like (LOC392067 Human)
   

   Use

   Tags

   8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. R777-E057 Hs.EIF4EBP2

   Plasmid

   #70341

   Purpose

   Gateway ORF clone of human EIF4EBP2 [NM_004096.4] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   EIF4EBP2 (EIF4EBP2 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R777-E058 Hs.EIF4EBP2-nostop

   Plasmid

   #70342

   Purpose

   Gateway ORF clone of human EIF4EBP2 [NM_004096.4] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   EIF4EBP2 (EIF4EBP2 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2

   Plasmid

   #202761

   Purpose

   Encodes four RGC specific transcription factors under the control of a TetOn promoter at the CLYBL safe harbor

   Depositor

   Karl Wahlin

   Article

   Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

   Insert

   NEUROG2-p2A-ATOH7-p2A-ISL1-p2A-POU4F2 (NEUROG2, ATOH7, ISL1, POU4F2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available Since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

   Plasmid

   #232880

   Purpose

   Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

   Depositor

   Paul DeCaen

   Article

   DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

   Insert

   ADP-ribosylation factor-like protein 13B (ARL13B Human)
   

   Use

   Lentiviral

   Tags

   GFP and RGECO1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

    Plasmid

    #232879

    Purpose

    Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    5-Hydroxytryptamine Receptor 6 (HTR6 Human)
    

    Use

    Lentiviral

    Tags

    RGECO1.1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-EGFP-DmPAN2-del246-249-dsRNAres_Y

    Plasmid

    #148034

    Purpose

    Insect Expression of DmPAN2-del246-249-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del246-249-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. psiCHECK3-EIF3B-88nt-A:U-Hairpin

    Plasmid

    #136034

    Purpose

    EIF3B (465-552) 3'UTR with the main hairpin mutated to A and U nucleotides only inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    EIF3B 3'UTR (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG416GAL10-HPeV_3C

    Plasmid

    #203465

    Purpose

    Expresses HPeV 3C protease from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    HPeV 3C protease (polyprotein gene )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGG-GST-TEV-NAP1 (WT)

    Plasmid

    #198034

    Purpose

    Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-shMcu-1

    Plasmid

    #181868

    Purpose

    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-MCU-EGFP

    Plasmid

    #181866

    Purpose

    Expresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV and Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-shMcu-2

    Plasmid

    #181867

    Purpose

    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSFDuet-1 ElonginB, ElonginC

    Plasmid

    #230999

    Purpose

    Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Inserts

    Elongin B (Elob Mouse)
    Elongin C (Eloc Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-MCU-tDimer

    Plasmid

    #181865

    Purpose

    Expresses tDimer-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV and Adenoviral

    Tags

    tDimer

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LSB-hsa-miR-30e-5p

    Plasmid

    #103419

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-30e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-30e-5p target (MIR30E Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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