Addgene

We narrowed to 1,214 results for: 5-Oct

Showing: 261 - 280 of 1214 results

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pCI-neo-HA-hAtg13

Plasmid

#22874

Depositor

Noboru Mizushima

Article

Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

Insert

autophagy-related genes 13 (ATG13 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2010

Availability

Academic Institutions and Nonprofits only
eGFP Y93H CC editing reporter

Plasmid

#198885

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGC)

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H AC editing reporter

Plasmid

#198883

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGA)

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP Y93H GC editing reporter

Plasmid

#198884

Purpose

Express eGFP fluorescence after Y93H correction and mCherry as transfection control

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

enhanced green fluorescent protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Y93H, Gly92(GGT-to-GGG)

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EK0302 SFFV-mCherry-SG(Bdnf.s2)SG-EGFP (FLP-IN)

Plasmid

#191179

Purpose

Negative control sensor in human cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s2:EGFP (negative control)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUltra-5HTP

Plasmid

#178042

Purpose

Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

Depositor

Han Xiao

Article

Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

Inserts

Modified ScTrpRS for 5HTP incoporation
suppressor tRNA for 5HTP incorporation

Use

Tags

Expression

Bacterial

Mutation

T107C, P254T, C255A

Promoter

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

Plasmid

#191146

Purpose

Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdnf.t1

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191183

Purpose

Expression of PPIB sensor in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:PPIB.s1:EGFP-3xMS2

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)

Plasmid

#191142

Purpose

Expression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s17d9b:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF4a

Plasmid

#67497

Purpose

Expression of shRNA targeting human ARF4a

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF4 shRNA (ARF4 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF4b

Plasmid

#67498

Purpose

Expression of shRNA targeting human ARF4b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF4 shRNA (ARF4 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF5a

Plasmid

#67499

Purpose

Expression of shRNA targeting human ARF5a

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF5 shRNA (ARF5 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-ARF5b

Plasmid

#67500

Purpose

Expression of shRNA targeting human ARF5b

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF5 shRNA (ARF5 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pETCK3 TGP-E

Plasmid

#236255

Purpose

Expresses TGP-E, a thermostable fluorescent protein

Depositor

Natasha DeVore

Article

Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

Insert

Thermal Green Protein Q66E (TGP-E)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

lac operon

Available since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCY5h

Plasmid

#160295

Purpose

Yeast CRISPR plasmid targeting the hphMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCY5n

Plasmid

#160296

Purpose

Yeast CRISPR plasmid targeting the natMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCY5k

Plasmid

#160294

Purpose

Yeast CRISPR plasmid targeting the kanMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CC0101 BFPmut-stop-Bdnf.t1 (pEAQ)

Plasmid

#191173

Purpose

An orthogonal trigger sequence used in plant experiments, derived from the 3' UTR of murine Bdnf

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdnf.t1 (plant)

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0209 CMV-TO-mCherry-SG(s70587)SG-EGFP (FLP-IN)

Plasmid

#191174

Purpose

Mammalian expression of EK0285 with CMV-tetO promoter instead of SFFV.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge