Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 441 results for: T7 rna polymerase

Clear keywords
Showing: 261 - 280 of 441 results

  1. pJin-264

    Plasmid
    #125717
    Purpose
    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19
    Depositor
    Bryan Dickinson
    Publication
    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
    Expression
    Mammalian
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125717-plasmid-map-sequence-id-244326

    Enlarge

  2. p7SL-neo-TET

    Plasmid
    #51285
    Purpose
    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Publication
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    7SL RNA (RN7SL2 Human)
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Promoter
    7SL upstream pollII enhancer
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51285-plasmid-map-sequence-id-78367

    Enlarge

  4. pJin-239

    Plasmid
    #125716
    Purpose
    ABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1
    Depositor
    Bryan Dickinson
    Publication
    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
    Expression
    Mammalian
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125716-plasmid-map-sequence-id-244343

    Enlarge

  12. pB2-neo-TET

    Plasmid
    #51287
    Purpose
    expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Publication
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    sine B2
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Promoter
    enhancer from 7SL gene
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51287-plasmid-map-sequence-id-78369

    Enlarge

  13. pRAV12

    Plasmid
    #67713
    Purpose
    Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification
    Depositor
    Robert Batey, Jeffrey Kieft
    Publication
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 RNA polymerase
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67713-plasmid-map-sequence-id-126588

    Enlarge

  18. mAC-POLR2A donor (Hygro)

    Plasmid
    #124496
    Purpose
    A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover
    Depositor
    Masato Kanemaki
    Publication
    Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
    Use
    CRISPR
    Tags
    mAID-mClover
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124496-plasmid-map-sequence-id-243723

    Enlarge

  19. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Publication
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47912-plasmid-map-sequence-id-68534

    Enlarge

  20. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Publication
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47911-plasmid-map-sequence-id-68528

    Enlarge

Showing: 261 - 280 of 441 results