Addgene

We narrowed to 1,466 results for: calcium

Showing: 261 - 280 of 1466 results

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Twitch-1 pcDNA3

Plasmid

#49812

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-1

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100849

Purpose

AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1b

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100850

Purpose

AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1b

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.Twitch2B.WPRE.SV40

Plasmid

#100039

Purpose

AAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch2B

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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Twitch-4 pcDNA3

Plasmid

#49533

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-4

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only
Rat Alpha2 delta-1 HA pMT2

Plasmid

#58729

Purpose

expression of HA tagged rat Alpha2 delta-1 in mamalian cells

Depositor

Annette Dolphin

Article

Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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Twitch-3 pcDNA3

Plasmid

#49532

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-3

Use

Tags

ECFP and cpCit174

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI

Plasmid

#160423

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

Plasmid

#100855

Purpose

AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

jRGECO1b

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.NES-jRGECO1b.WPRE.SV40

Plasmid

#100856

Purpose

AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

jRGECO1b

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV GFAP jGCaMP7b

Plasmid

#171118

Purpose

Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

Depositor

Thomas Oertner

Article

Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

Insert

jGCaMP7b

Use

AAV

Tags

6 His Tag, T7 Tag, and Xpress Tag

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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piGECI-NES-N1

Plasmid

#160422

Purpose

Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Twitch-2C pcDNA3

Plasmid

#104501

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pShuttle CMV RGECO-TnI

Plasmid

#124642

Purpose

Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination

Depositor

Paul Robinson, Matthew Daniels, Hugh Watkins

Article

Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

Insert

RGECO-TnI (TNNI3 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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G-GECO1.2-Orai1Y80E

Plasmid

#73565

Purpose

Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1.2-Orai1Y80E (ORAI1 Human, Synthetic)

Use

Tags

G-GECO1.2

Expression

Mammalian

Mutation

changed tyrosine 80 of Orai1 to glutamate

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Twitch-3 pRSETB

Plasmid

#48204

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-3A

Use

Tags

6xHIS, cpVenusCD, and mCerulean3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only
cacna2d1 R241A pMT2

Plasmid

#206110

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

R241A

Promoter

Ad MLP/TPL/SV40

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 R241A HA pMT2

Plasmid

#206111

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

R241A

Promoter

Ad MLP/TPL/SV40

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 D122A pcDNA3

Plasmid

#206068

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS W391A pcDNA3

Plasmid

#206077

Purpose

Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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