Addgene

We narrowed to 1,125 results for: cas12

Showing: 261 - 280 of 1125 results

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pCH61 (multiAsCas12a-KRAB piggyBac)

Plasmid

#217332

Purpose

piggyBac expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

R1226A/E174R/S542R/K548R

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV enAsCas12f-YHAM-sgRNA_v7

Plasmid

#208356

Purpose

Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-YHAM

Use

Tags

Expression

Mammalian

Mutation

F48Y/S188H/V232A/E316M

Promoter

CMV

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-FNLDTA

Plasmid

#195340

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis Tag and MBP, TEV cleavage site, 6x His Tag

Expression

Bacterial

Mutation

F208W, N524V, L795I, D868V, T874S, A1015E

Promoter

T7 Promoter

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-pML-1B_6xHis-Cas12m

Plasmid

#192282

Purpose

MmCas12m fused to His-tag for protein purification

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12m

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AsCas12a-Plus-mCherry

Plasmid

#191231

Purpose

To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

Depositor

Zhili Rong

Article

Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

Insert

AsCas12a-Plus-P2A-mCherry

Use

CRISPR

Tags

Expression

Mutation

E174R, R951K, R955A

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCE046-SiT-Cas12a-[Repr]

Plasmid

#128132

Purpose

Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Repr]-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-NLS-BhCas12b_v4

Plasmid

#158615

Purpose

For protein expression of BhCas12b_v4

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

NLS-BhCas12b_v4

Use

Tags

6xHIS-TwinStrep-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e-V106W

Plasmid

#193646

Purpose

Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e-V106W, EGFP

Use

Tags

Expression

Mammalian

Mutation

TadA*8e-V106W

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCI108-SiT-Cas12a-[Cond]

Plasmid

#128407

Purpose

Conditionally express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCI118-SiT-Cas12a-[Ind]

Plasmid

#128406

Purpose

Inducible expression of both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-enCas12a-ABE8e-V106W

Plasmid

#193653

Purpose

Expression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-TadA*8e-V106W, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, TadA*8e-V106W

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1-v5.1+T1-empty

Plasmid

#204977

Purpose

AsCas12f1-v5.1+T1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nature Catalysis (2023) 6, 695–709

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

N70Q/K103R/A104R/S118A/D364R

Promoter

PCMV,U6

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-N57G

Plasmid

#193639

Purpose

Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-N57G, EGFP

Use

Tags

Expression

Mammalian

Mutation

hAPOBEC3A-N57G

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-Y130F

Plasmid

#193640

Purpose

Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-Y130F, EGFP

Use

Tags

Expression

Mammalian

Mutation

hAPOBEC3A-Y130F

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pZ148-BhCas12b-sgRNA-scaffold

Plasmid

#122448

Purpose

Expresses sgRNA for BhCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BhCas12b-sgRNA-scaffold, CMV-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCH62 (denAsCas12a-KRAB piggyBac)

Plasmid

#217333

Purpose

piggyBac expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

D908A/E174R/S542R/K548R

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-AsCas12f-2NLS

Plasmid

#171613

Purpose

NLS-AsCas12f1-NLS heterologous expression plasmid for RNP

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

NLS-AsCas12f1-NLS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-AsCas12a_crRNA-site4 (MSP3511)

Plasmid

#160139

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)

Use

In vitro transcription of sgrna from t7 promoter

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-opCas12a-Ultra-6xNLS

Plasmid

#209024

Purpose

Lentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

opCas12a-Ultra-6xNLS-Flag-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

FLAG and MYC

Expression

Mammalian

Mutation

E174R/M537R/S542R/F870L

Promoter

EF-1a

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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