We narrowed to 22,022 results for: his

1. miniTurbo-His6_pET21a

   Plasmid

   #107178

   Purpose

   expresses His6-tagged miniTurbo in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-hISG15

   Plasmid

   #12447

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   ISG15 fragment (ISG15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ISG15 up to LRLRGG

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1-melHis10TEV

   Plasmid

   #159428

   Purpose

   empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Use

   Tags

   melittin signal sequence (removed in vivo by sign…

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTrcHIS-wt7D12

   Plasmid

   #125268

   Purpose

   Expresses wild type 7D12 anti-EGFR nanobodies

   Depositor

   Angus Johnston

   Article

   Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

   Insert

   7D12 nanobody
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Trc

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet-1∆HisTag_hNMT_Nef

   Plasmid

   #66078

   Purpose

   Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

   Depositor

   Dieter Willbold

   Article

   Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

   Inserts

   NMT (NMT1 Human)
   Nef
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Deletion of first 80 amino acids

   Promoter

   T7-lacO

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHis-hTG2

   Plasmid

   #100719

   Purpose

   Expression of human transglutaminase 2 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

   Insert

   TGM2 (TGM2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   silent mutation Arg580 (AGG--CGT) to enhance expr…

   Promoter

   T7

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-8xHis-Ub

   Plasmid

   #107392

   Purpose

   Mammalian expression 8xHis-Ub

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   8 tandem repeats of 6xHis-Ub (See comments below)
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p6XHis_NLS-SaCas9

   Plasmid

   #101086

   Purpose

   Express SaCas9 in bacteria with a 6xHis tag for purification

   Depositor

   Rick Tarleton

   Article

   Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

   Insert

   CRISPR-associated protein Cas9/Csn1
   

   Use

   CRISPR

   Tags

   TrxA-6xHis-STag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a His6HuR

   Plasmid

   #135943

   Purpose

   Expresses HuR in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-HisMBP

    Plasmid

    #11085

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac (lactose/IPTG inducible)

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLBH559_Tet-HisCas14a1Locus

    Plasmid

    #112502

    Purpose

    Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTrcHIS-7D12Gln13TAG

    Plasmid

    #125264

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    Gln13TAG 7D12
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. His6TEV-UbG76C

    Plasmid

    #75299

    Purpose

    Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    human Ubiquitin mutant G76 to C
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Glycine 76 to Cystine

    Promoter

    T7

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. WDV-Echistatin

    Plasmid

    #226985

    Purpose

    Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

    Depositor

    Wendy Gordon

    Article

    Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

    Inserts

    WDV (repA )
    Echistatin
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. hisSUMO-CTCF(F1-F9)

    Plasmid

    #102859

    Purpose

    Express DNA binding domain of mouse CTCF in E. coli

    Depositor

    Gary Stormo

    Article

    Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

    Insert

    CTCF (Ctcf Mouse)
    

    Use

    Tags

    hisSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGS140_6xHis-EGFP-I3-01

    Plasmid

    #211889

    Purpose

    Bacterial expression of I3-01

    Depositor

    Iain Cheeseman

    Insert

    I3-01
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b_pScaC-SNAP_6xHis

    Plasmid

    #228835

    Purpose

    E. coli expression of the SNAP-tagged ScaC passenger domain

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    SNAPtag-TEV-6xHis

    Expression

    Bacterial

    Mutation

    aa 33-223

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBADZ-HisCre

    Plasmid

    #111187

    Purpose

    Helper plasmid containing Cre-recombinase gene, which is expressed in DH10MultiBac cells

    Depositor

    Tim Richmond

    Article

    Berger et al Nat Biotechnol. 2004 Dec;22(12):1583-7.

    Insert

    Cre recombinase
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac1-His10FLAG

    Plasmid

    #157736

    Purpose

    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

    Depositor

    Debra Hansen

    Article

    Olson et al Protein Expr Purif. 2021 May 7:105890. d

    Type

    Empty backbone

    Use

    Tags

    His10 + 3xFLAG, enterokinase-cleavable

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P1-N-His

    Plasmid

    #119755

    Purpose

    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    10xHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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