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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Publication

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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sortase A pentamutant (eSrtA) in pET29

Plasmid

#75144

Purpose

evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

Depositor

David Liu

Publication

Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

Insert

sortase A pentamutant

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T. Deleted amino a…

Promoter

T7

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

Enlarge
pET-28b-Cas9-His

Plasmid

#47327

Purpose

For in vitro expression and purification of Cas9 protein

Depositor

Alex Schier

Publication

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR

Tags

His

Expression

Bacterial

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

Enlarge
pET-28b-Cas9-His

Plasmid

#47327

Purpose

For in vitro expression and purification of Cas9 protein

Depositor

Alex Schier

Publication

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR

Tags

His

Expression

Bacterial

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

Enlarge
GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Publication

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p65 (RELA Human)

Tags

EGFP

Expression

Mammalian

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Publication

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p65 (RELA Human)

Tags

EGFP

Expression

Mammalian

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

Enlarge
GFP-rab7 WT

Plasmid

#12605

Depositor

Richard Pagano

Publication

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

73 Articles

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GFP-rab7 WT

Plasmid

#12605

Depositor

Richard Pagano

Publication

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

73 Articles

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EGFP-alphasynuclein-A53T

Plasmid

#40823

Depositor

David Rubinsztein

Publication

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNCA (SNCA Human)

Tags

EGFP

Expression

Mammalian

Mutation

A53T (aggregates more rapidly than the wild-type …

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

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EGFP-alphasynuclein-A53T

Plasmid

#40823

Depositor

David Rubinsztein

Publication

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNCA (SNCA Human)

Tags

EGFP

Expression

Mammalian

Mutation

A53T (aggregates more rapidly than the wild-type …

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

38 Articles

Enlarge
pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Publication

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

101 Articles

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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Publication

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

101 Articles

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gRNA_AAVS1-T2

Plasmid

#41818

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA_AAVS1-T2

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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gRNA_AAVS1-T2

Plasmid

#41818

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA_AAVS1-T2

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

Enlarge
phH1-gRNA

Plasmid

#53186

Purpose

Expresses the S. pyogenes sgRNA from the H1 promoter

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human H1

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

Enlarge
phH1-gRNA

Plasmid

#53186

Purpose

Expresses the S. pyogenes sgRNA from the H1 promoter

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human H1

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

Enlarge
mCh-alpha-tubulin

Plasmid

#49149

Purpose

expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

Depositor

Gia Voeltz

Publication

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

alpha tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

Enlarge
mCh-alpha-tubulin

Plasmid

#49149

Purpose

expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

Depositor

Gia Voeltz

Publication

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

alpha tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

Enlarge
pAdTrack-CMV

Plasmid

#16405

Purpose

Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

Enlarge
pAdTrack-CMV

Plasmid

#16405

Purpose

Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

Enlarge