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Plasmid#113214Purposesafe haven knock-in plasmid for the act5 locus, C-terminal mTurquoise2 taggingDepositorTypeEmpty backboneUseSafe haven knock-in plasmid for the act5 locus, c…TagsmTurquoise2ExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only
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p11-3’_8xN_PAM-site1 (RTW554)
Pooled Library#160132PurposePlasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)DepositorSpeciesSyntheticUseCRISPRAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p11-5’_10xN_PAM-site4 (RTW574)
Pooled Library#160135PurposePlasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)DepositorSpeciesSyntheticUseCRISPRAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p11-3’_8xN_PAM-site2 (RTW555)
Pooled Library#160133PurposePlasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)DepositorSpeciesSyntheticUseCRISPRAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHoss1
Plasmid#63158PurposeSuicide plasmid for efficient gene mutation in Gram-positive bacteriaDepositorInsertanti-secY-Pxyl/tetO-tetR
UseSuicide plasmid for gram-positive bacteriaTagsExpressionMutationPromoterAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep
Plasmid#213542PurposeAAV viral vector packaging plasmid that expresses CoV2DepositorInsertCoV2 Orf3a-2xStrep (ORF3a Human, Severe acute respiratory syndrome coronavirus 2)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1993 - pAAV EF1a CoV2 protE-2xStrep
Plasmid#213543PurposeAAV viral vector packaging plasmid that expresses CoV2DepositorInsertCoV2 protE-2xStrep (E Human, Severe acute respiratory syndrome coronavirus 2)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.Cre.WPRE.hGH
Plasmid#105550PurposeAAV expression of Cre from GFAP promoterDepositorHas ServiceAAV PHP.eB and AAV5InsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterGFAPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GfaABC1D.PI.Cre.SV40
Plasmid#105603PurposeAAV expression of Cre from GfaABC1D promoterDepositorInsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV.TBG.PI.Cre.rBG
Plasmid#107787PurposeAAV vector expressing Cre from TBG promoterDepositorHas ServiceAAV8InsertCre
UseAAVTagsExpressionMammalianMutationPromoterTBGAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-Cre
Plasmid#182736PurposeCre expression under the control of CamKII promoterDepositorInsertCre
UseAAVTagsExpressionMutationPromoterCamKIIAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.rTH.PI.Cre.SV40
Plasmid#107788PurposeAAV vector expressing Cre from rTH promoterDepositorHas ServiceAAV Retrograde and AAV9InsertCre
UseAAVTagsExpressionMammalianMutationPromoterrTHAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC32 [TU#839]
Plasmid#16079DepositorInsertmec-2 intron 9
UseTagsYFPExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
AV0.1-CWS-Cre-tdT
Plasmid#192876Purposecoexpression of Cre and tdTDepositorInsertCre-P2A-tdTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AV0.1-CWS-Cre-mCherry
Plasmid#192877Purposecoexpression of Cre and mCherryDepositorInsertCre-T2A-mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX552-GFP
Plasmid#107023PurposepX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.DepositorInsertGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOttc438 - pAAV EF1a CRE ERT2 bGHpA
Plasmid#210415PurposeAAV viral vector packaging plasmid that expresses tamoxifen-regulated Cre recombinaseDepositorInsertCre-ERT2
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP13:5325-5925_BV
Plasmid#234374PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp13_SARS2
UseBaculovirus expresssionTagsMHHHHHHSSGRENLYFQGExpressionMutationPromoterPolyhedrinAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR/hBIG1
Plasmid#226251PurposeEntry plasmid of hBIG1 for Gateway systemDepositorInsertBIG1 (ARFGEF1 Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecBCD
Bacterial Strain#176583PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.DepositorBacterial ResistanceChloramphenicolAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only