We narrowed to 13,736 results for: TIM;

1. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

   Plasmid

   #20946

   Purpose

   3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   halorhodopsin
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAJM.969

   Plasmid

   #108537

   Purpose

   MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   MphRAM + EryR + PMph-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   MphR: N94D

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CeNL-Clathrin_pEGFP

   Plasmid

   #89540

   Purpose

   Cyan color variant of bright luminescent protein, fused to clathrin light chain

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   CeNL
   

   Use

   Tags

   Clathrin light chain

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

   Plasmid

   #175524

   Purpose

   Co-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Beta4Gal-HaloTag7 and Lyn11-SNAPf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-CaMKIIbeta(K43R)

   Plasmid

   #21225

   Depositor

   Tobias Meyer

   Article

   Shen et al Science. 1999 Apr 2. 284(5411):162-6.

   Insert

   CaMKIIbeta(K43R) (Camk2b Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CaMKIIbeta(K43R) Lab plasmid CF 0003.

   Promoter

   Available since

   Sept. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-QFGal4-SV40pA

   Plasmid

   #155126

   Purpose

   In vitro transcription of QFGal4 mRNA

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QFGal4
   

   Use

   Expression in zebrafish

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-C1-CAMKIIalpha-K42R

   Plasmid

   #21221

   Depositor

   Tobias Meyer

   Article

   Shen et al Science. 1999 Apr 2. 284(5411):162-6.

   Insert

   CAMKIIalpha-K42R (Camk2a Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K42R (Lab plasmid KS 0007).

   Promoter

   Available since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pRSETb-ffDronpa

   Plasmid

   #78182

   Purpose

   Expresses ffDronpa E. coli strains

   Depositor

   Peter Dedecker

   Article

   Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

   Insert

   ffDronpa
   

   Use

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMito-Electra1

   Plasmid

   #179481

   Purpose

   Mitochondrial labeling with blue fluorescent protein Electra1 fused to partial COX8A

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   COX8A-Electra1
   

   Use

   Tags

   Electra1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. HBT_pcoCASphi_version2

    Plasmid

    #197932

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    2xNLS-2xFLAG

    Expression

    Plant

    Mutation

    Promoter

    35SPPDK promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mMaroon-RTK-mMaroon

    Plasmid

    #84359

    Purpose

    FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-Rtk--mMaroon
    

    Use

    Tags

    mMaroon flanking Rtk domain on N and C terminus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-PtH2B

    Plasmid

    #133390

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. HaloTag-2xrGBD

    Plasmid

    #176109

    Purpose

    Visualization of active Rho

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    rhotekin (Rtkn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEntr1a-wGBD-mTurquoise2-wGBD

    Plasmid

    #176136

    Purpose

    Visualization of active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    WAS (WAS Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS-H2B-EYFP

    Plasmid

    #53746

    Purpose

    contains fusion of human histone H2B to EYFP in pCS2+ vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLV_Lyn11-mTurquoise2-iLID

    Plasmid

    #161000

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    Lyn11-mTurquoise2-iLID
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET14-GFP15_Encap

    Plasmid

    #86407

    Purpose

    Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 15aa CLP tag
    Encapsulin
    

    Use

    Tags

    6x Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYZ146

    Plasmid

    #98406

    Purpose

    Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.leu1 (leu1 Fission Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1-CaMKIIbeta(A303R)

    Plasmid

    #21224

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CaMKIIbeta(A303R) (Camk2b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CaMKIIbeta(A303R) (Lab plasmid CF 0002)

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUCBB-pT7-eGFP

    Plasmid

    #32552

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    pT7

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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