We narrowed to 16,598 results for: Jun

1. pJH857

   Plasmid

   #172175

   Purpose

   PylB overexpression and purification

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJH202

   Plasmid

   #172150

   Purpose

   Accessory Plasmid for PACE of pylBCD variants

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Ppsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gIII(P29*); 32A pylS variant

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJH223

   Plasmid

   #172151

   Purpose

   pylBCD variant in pCDF backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/lac (pylBCD 34B_sub-pop5); LacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PylB(N61S, E178K, E251D) PylD(A252V)

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJH224

   Plasmid

   #172152

   Purpose

   pylBCD variant in pCDF backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/lac (pylBCD 40C_sub-pop6); LacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PylB(N61S, E175K, E178K)

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJH463

   Plasmid

   #172155

   Purpose

   pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Para (pylBCD 36A_sub-pop1); AraC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PylB(N61S, E122K, E178K, G188R)

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJH573

   Plasmid

   #172157

   Purpose

   pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Para (Untagged pylBCD); AraC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon-optimized Methanosarcina acetivorans pylBCD

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH574

   Plasmid

   #172158

   Purpose

   pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Para (pylBCD 36A_sub-pop2); AraC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PylB(N61S, E84K, E122K) PylC(E316D)

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJH575

   Plasmid

   #172159

   Purpose

   pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Para (pylBCD 34B_sub-pop3); AraC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Sumo(S6L) PylB(N61S, E178K, G188R, E251D) PylD(A2…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJH576

   Plasmid

   #172160

   Purpose

   pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Para (pylBCD 34B_sub-pop4); AraC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PylB(N61S, E175K, E178K, E251D) PylD(A252V)

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJH19

    Plasmid

    #172141

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.1TAG, luxAB; Plpp pylS; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gIII(P29*)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJH60

    Plasmid

    #172142

    Purpose

    Microscopy inclusion body assay

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    pCDF.CD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PylCD partial operon (PylB deleted to assess impa…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH77

    Plasmid

    #172145

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.3TAG, luxAB; Plpp pylS; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gIII(P29*, P83*, Y184*)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJH123

    Plasmid

    #172146

    Purpose

    Microscopy inclusion body assay

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    pCDF.B.(SD4)CD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PylBCD operon (RBS inserted for PylC)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJH126

    Plasmid

    #172147

    Purpose

    Microscopy inclusion body assay

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    pCDF.SUMO-B.(SD4).CD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PylBCD operon (SUMO tag inserted for PylB; RBS in…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ssh1 gRNA#2

    Plasmid

    #163395

    Purpose

    Cas9-mediated knockout of Ssh1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    ssh1 (Ssh1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Ssh1 gRNA#1

    Plasmid

    #163394

    Purpose

    Cas9-mediated knockout of Ssh1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    ssh1 (Ssh1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ssh1 gRNA#3

    Plasmid

    #163396

    Purpose

    Cas9-mediated knockout of Ssh1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    ssh1 (Ssh1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHIS8-3_DL121

    Plasmid

    #171953

    Purpose

    Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

    Depositor

    Kimberly Reynolds

    Article

    McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

    Insert

    Fusion of ecDHFR and asLOV2 at position 121 of DHFR
    

    Use

    Tags

    8-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBL-2

    Plasmid

    #162769

    Purpose

    Minimal backbone with AmpR

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Molecular cloning

    Tags

    Expression

    Mutation

    Promoter

    no

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACYCDuet_DL121_WTTS

    Plasmid

    #171954

    Purpose

    Selection vector for chimeric fusion of ecDHFR and asLOV2.

    Depositor

    Kimberly Reynolds

    Article

    McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

    Inserts

    Fusion of ecDHFR and asLOV2 at position 121 of DHFR
    Thymidylate Synthase
    

    Use

    Tags

    8-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge