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Flag-SENP1

Plasmid

#17357

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-pvc10

Plasmid

#243750

Purpose

For affinity purification of spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Ring2-FLAG-AID-mClover

Plasmid

#246226

Purpose

Expression of Ring2-FLAG-AID

Depositor

Hengbin Wang

Insert

Ring2 (RNF2 Human)

Use

CRISPR

Tags

Flag-AID-mClover

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET A-mEosEM-E

Plasmid

#226336

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; mEosEM-E for prokaryotic expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Insert

mEosEM-E

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SENP1m

Plasmid

#17358

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R630L, K631M

Available Since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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pH3U3-mcs

Plasmid

#12609

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Multiple cloning site in His3 Ura3 reporter plasmid

Expression

Bacterial

Available Since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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PX408 Francisella tularensis subsp. novicida Cas9

Plasmid

#68705

Purpose

Francisella tularensis subsp. novicida Cas9

Depositor

Feng Zhang

Insert

FtCas9

Use

CRISPR

Tags

HA-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSCC2

Plasmid

#39993

Depositor

Paul Modrich

Article

Cheng et al J Biol Chem. 1984 Sep 25;259(18):11571-5

Insert

EcoRI endonuclease & EcoRI methylase

Tags

none

Expression

Bacterial

Mutation

none

Promoter

n/a

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pAC1817_pCR8-gSMN2-EX

Plasmid

#118651

Purpose

Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-EX

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

Plasmid

#68337

Purpose

Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

Depositor

Feng Zhang

Insert

St3Cas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CBh

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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EB2316

Plasmid

#87753

Purpose

pkaiBC::EYFP cloned between XbaI and XhoI of EB2065

Depositor

Erin O'Shea

Article

Zheng et al Cell Rep. 2017 Apr 18;19(3):497-504. doi

Insert

pkaiBC::EYFP

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAC1808_pmax-RBFOX1N-FRB-C

Plasmid

#118641

Purpose

Transient transfection; Expresses RBFOX1N-FRB-C; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBFOX1N-FRB-C

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1819_pCR8-DR-SMN2-2-DR

Plasmid

#118653

Purpose

Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-SMN2-2-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1809_pmax-RBM38-FRB

Plasmid

#118642

Purpose

Transient transfection; Expresses RBM38-FRB; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBM38-FRB

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1810_pmax-FRB-RBM38

Plasmid

#118643

Purpose

Transient transfection; Expresses FRB-RBM38; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

FRB-RBM38

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1818_pCR8-gRG6-SA

Plasmid

#118652

Purpose

Transient transfection; Expressed RG6-SA CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gRG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1820_pCR8-DR-RG6-SA-DR

Plasmid

#118654

Purpose

Transient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

DR-RG6-SA-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1822_pCR8-dCasRx

Plasmid

#118656

Purpose

Gateway Donor; dCasRx ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Use

CRISPR

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EB2315

Plasmid

#87752

Purpose

BbbJ23119::EYFP cloned between XbaI and XhoI of EB2065

Depositor

Erin O'Shea

Article

Zheng et al Cell Rep. 2017 Apr 18;19(3):497-504. doi

Insert

BbbJ23119::EYFP

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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PX379 Legionella pneumophila str. Paris Cas9

Plasmid

#68316

Purpose

Legionella pneumophila str. Paris Cas9

Depositor

Feng Zhang

Insert

LpCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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PX391 Sphaerochaeta globus str. Buddy Cas9

Plasmid

#68328

Purpose

Sphaerochaeta globus str. Buddy Cas9

Depositor

Feng Zhang

Insert

SgCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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EB2317

Plasmid

#87754

Purpose

tetR-mTurqoise2 cloned between BamHI and EcoRI of pAM2991

Depositor

Erin O'Shea

Article

Zheng et al Cell Rep. 2017 Apr 18;19(3):497-504. doi

Insert

TetR-mTurq2

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pVP311

Plasmid

#222070

Purpose

MultiGreen 2.0 AB level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP312

Plasmid

#222071

Purpose

MultiGreen 2.0 BC level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP313

Plasmid

#222072

Purpose

MultiGreen 2.0 CD level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP314

Plasmid

#222073

Purpose

MultiGreen 2.0 DE level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP315

Plasmid

#222074

Purpose

MultiGreen 2.0 EF level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP316

Plasmid

#222075

Purpose

MultiGreen 2.0 FG level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP078

Plasmid

#222030

Purpose

MultiGreen 2.0 AB level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP079

Plasmid

#222031

Purpose

MultiGreen 2.0 BC level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge