We narrowed to 9,607 results for: FIE

1. pWzyE-GST-distrepII

   Plasmid

   #196666

   Purpose

   Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   GST and TEV-cleavable di-strep tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWzyE-strep-mClover3

   Plasmid

   #196668

   Purpose

   Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Use

   Tags

   TEV-cleavable di-strep tag II and mClover3

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-ExSYTE.H64A

   Plasmid

   #216262

   Purpose

   inactive ExSYTE.H64A (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

   Depositor

   Susumu Tomita

   Article

   Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

   Insert

   ExSYTE P2A EGFP - Rat DTE
   

   Use

   AAV

   Tags

   Expression

   Mutation

   H64A mutation in each oTetR

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast CG1358

   Plasmid

   #218541

   Purpose

   Drosophila CG1358 cDNA (human codon optimized)

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   CG1358 (HisT Fly)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. plentiCRISPR-v2 Puro SPNS1_sg2

   Plasmid

   #218531

   Purpose

   sgRNA targeting human SPNS1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SPNS1 (SPNS1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. plentiCRISPR-v2 Puro SPNS1_sg1

   Plasmid

   #218530

   Purpose

   sgRNA targeting human SPNS1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SPNS1 (SPNS1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-Nova1-GFP

   Plasmid

   #217033

   Purpose

   Overexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neurons

   Depositor

   Chaolin Zhang

   Article

   Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

   Insert

   Nova1 (Nova1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET151-GyrA_F164R238

   Plasmid

   #216230

   Purpose

   S. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coli

   Depositor

   Eric Brown

   Article

   French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

   Insert

   DNA gyrase
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Changed arginine 238 to serine, phenylalanine 168…

   Promoter

   T7

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET151-GyrB_A467

   Plasmid

   #216234

   Purpose

   S. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coli

   Depositor

   Eric Brown

   Article

   French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

   Insert

   DNA gyrase
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Changed alanine 467 to valine

   Promoter

   T7

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET151-GyrB_T522

    Plasmid

    #216235

    Purpose

    S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed threonine 522 to isoleucine

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR4 genomic A. thaliana PLDGAMMA3

    Plasmid

    #209188

    Purpose

    pENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    AT4G11840 (PLDGAMMA3 Mustard Weed)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUAST ɑSynWT-EGFP

    Plasmid

    #201249

    Purpose

    allows the expression of the insert in Drosophila melanogaster

    Depositor

    Pedro Domingos

    Article

    Pocas et al Hum Mol Genet. 2015 Apr 1;24(7):1898-907

    Insert

    ɑ-Synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x Linker-GPI-PSS

    Plasmid

    #194751

    Purpose

    To fuse your protein of interest to the C-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPO794

    Plasmid

    #195303

    Purpose

    pBAD322-MycaIDCas-ΔCas6-RGR-PaqCI, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked PaqCI entry site.

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    McCAST Cascade Δcas6 and ribozyme flanked entry site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose inducible arapBAD

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-TBG-Cre-sgArid1a

    Plasmid

    #192165

    Purpose

    AAV-TBG-Cre-sgArid1a

    Depositor

    Sidi Chen

    Article

    Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-TBG-Cre-sgKmt2d

    Plasmid

    #192164

    Purpose

    AAV-TBG-Cre-sgKmt2d

    Depositor

    Sidi Chen

    Article

    Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pv6_CBX7_2xChromo-W35A

    Plasmid

    #179397

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo(W35A)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_2xChromo(W35A)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Chromodomain W35A

    Promoter

    CAGGS

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pv6_CBX1_2xChromo-W42A

    Plasmid

    #179394

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo(W42A)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo(W42A)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Chromodomain W42A

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pv7_MBD1_MBD_noEGFP

    Plasmid

    #179406

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1_MBD
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pv7_CBX1_2xChromo_noEGFP

    Plasmid

    #179395

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo_noEGFP
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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