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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,228 results for: Streptococcus

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Showing: 2801 - 2820 of 3228 results

  1. pNRCreBle

    Plasmid
    #234431
    Purpose
    Picochlorum celeri ShBle insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removal
    Depositor
    Matthew Posewitz
    Article
    Burch et al Front Microbiol. 2025 Jul 14;16:1588625.
    Insert
    Cre recombinase
    Use
    CRISPR, Cre/Lox, and Synthetic Biology ; Green al…
    Mutation
    Picochlorum celeri endoglucanase intron insertion
    Promoter
    P celeri nitrate reductase
    Available Since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234431-plasmid-map-sequence-id-464905

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  2. iPEmax-WT

    Plasmid
    #239385
    Purpose
    For inverse prime editor using Cas9-R221K-N394K in HEK294T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    Cas9-R221K-N393K, M-MLV-RT
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS, c-myc NLS
    Expression
    Mammalian
    Mutation
    R221K, N394K in Cas9
    Promoter
    CMV
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239385-plasmid-map-sequence-id-481781

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  3. iPEmax-D10A

    Plasmid
    #239386
    Purpose
    For inverse prime editor using nCas9-D10A-R221K-N394K in HEK295T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    nCas9-D10A-R221K-N394K, M-MLV-RT
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS, c-myc NLS
    Expression
    Mammalian
    Mutation
    D10A, R221K, N394K in Cas9
    Promoter
    CMV
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239386-plasmid-map-sequence-id-481780

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  9. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

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  10. pENTR-Cas9-TagBFP

    Plasmid
    #236725
    Purpose
    Cloning vector Cas9-TagBFP
    Depositor
    David Bersten
    Insert
    Cas9-TagBFP
    Use
    CRISPR
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236725-plasmid-map-sequence-id-474213

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  11. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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Showing: 2801 - 2820 of 3228 results