We narrowed to 8,385 results for: Dos

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pTRE Tight ArchT-GFP

Plasmid

#79627

Purpose

Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

Depositor

Yasunori Hayashi

Article

Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

Insert

ArchT

Tags

EGFP

Expression

Mammalian

Mutation

ArchT is a more sensitive version of Arch (archae…

Promoter

Tet responsive Ptight promoter

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only
FLAG-MKK6 (S207E,T211E)-pcw107

Plasmid

#64625

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K6 (MAP2K6 Human)

Use

Lentiviral

Tags

FLAG

Mutation

S207E,T211E

Promoter

PGK

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only
SmoM2 (W535L)-pcw107

Plasmid

#64627

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

SMO (SMO Human)

Use

Lentiviral

Mutation

W535L

Promoter

PGK

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only
Gli2 truncation-pcw107-V5

Plasmid

#64626

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

GLI2 (truncation mutant) (GLI2 Human)

Use

Lentiviral

Tags

V5

Mutation

truncated

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
BCL-XL-pcw107-V5

Plasmid

#64631

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

BCL2L1 (transcript variant 1) (BCL2L1 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
Caspase-3 (C163A)-pcw107-V5

Plasmid

#64633

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CASP3 (transcript variant beta) (CASP3 Human)

Use

Lentiviral

Tags

V5

Mutation

C163A

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pCX4-KLF4

Plasmid

#36118

Depositor

Steven Dowdy

Article

Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

Insert

kruppel-like factor 4 (KLF4 Human)

Use

Retroviral

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPBAD-yhjH

Plasmid

#111613

Purpose

Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

Depositor

Tim Tolker-Nielsen

Article

Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

Insert

yhjH

Expression

Bacterial

Mutation

A52V

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

Plasmid

#82705

Purpose

AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

Depositor

Jonathan Ploski

Article

de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

Insert

U6/TO gRNA Expression Cassette Containing Tet2 gRNA

Use

AAV and CRISPR

Tags

CMV promoter driving expression of TetR-P2A-GFP-K…

Expression

Mammalian

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBF_HsPepT1

Plasmid

#73943

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag

Depositor

Simon Newstead

Article

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT1 (SLC15A1 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pBF_HsPepT2

Plasmid

#73944

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag

Depositor

Simon Newstead

Article

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT2 (SLC15A2 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
HcRed-pcw107-V5

Plasmid

#64647

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
Cbh_v5 AAV-saCBE N-terminal

Plasmid

#137182

Purpose

AAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-saCBE N-terminal

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S

Plasmid

#106177

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

hSynapsin

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pF CAG luc IRES neo

Plasmid

#98294

Purpose

For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Inserts

Firefly luciferase
IRES
Neomycin resistance gene (NeoR)

Use

Lentiviral and Luciferase

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK7_WT

Plasmid

#82925

Purpose

Gateway Donor vector containing MAPK7, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_002749.3 (MAPK7 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFB2G

Plasmid

#195290

Purpose

Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mTagBFP

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307-IBV-3CL

Plasmid

#160288

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL

Plasmid

#160284

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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