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PFD1130w-bio-His

Plasmid

#50823

Purpose

Expresses enzymatically monobiotinylated full length PFD1130w

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFD1130w

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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SERA4-bio-His

Plasmid

#50816

Purpose

Expresses enzymatically monobiotinylated full length SERA4

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA4

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pBIG2ab

Plasmid

#80616

Purpose

pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFBMBD4-C-GFP (pc1567)

Plasmid

#211714

Purpose

Expresses fl. MBD4 GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD4

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-H2B-mScarlet-I

Plasmid

#182297

Purpose

Localization of mScarlet-I fluorescent protein to histone 2B

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

mScarlet-I

Tags

mScarlet-I

Expression

Mammalian

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-tMCK-ER-TurboID

Plasmid

#149412

Purpose

Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

tMCK

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNXF-1_K

Plasmid

#146740

Purpose

Mammalian Expression of HsNxf-1

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

HsNxf-1 (NXF1 Human)

Expression

Mammalian

Mutation

S144G relative to NM_006362.4

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-PUb

Plasmid

#52891

Purpose

Aedes aegypti polyubiquitin promoter driving firefly luciferase

Depositor

Zach Adelman

Article

Anderson et al Insect Mol Biol. 2010 Aug;19(4):441-9. d

Insert

polyubiquitin promoter

Use

Luciferase

Promoter

Ae aegypti polyubiquitin

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_neomycin

Plasmid

#65306

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Tags

KDEL motif and Signal Sequence

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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HSA_3b*_AP

Plasmid

#238899

Purpose

Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

Recombinant Human Serum Albumin (HSA) 3b*

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(1-710)

Plasmid

#228831

Purpose

Insect cell expression of truncated BICD2 (aa 1-710)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 1-710

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 V5-DEST 3XFlag-SRSF3

Plasmid

#46736

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

SRSF3 (SRSF3 Human)

Tags

3x Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChR2(H134R)-GFP

Plasmid

#58880

Purpose

AAV expression of ChR2(H134R)-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Has Service

AAV Retrograde and AAV8

Insert

ChR2(H134R)-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

H134R

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-GCaMP6s

Plasmid

#166606

Purpose

Encodes Cre-dependent GCaMP6s under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

GCaMP6s

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1e

Plasmid

#80615

Purpose

pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-LAMP1-iLID-Halo

Plasmid

#247296

Purpose

Mammalian expression of LAMP1-iLID-Halo fusion for light-induced recruitment to late endosomes/lysosomes.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

LAMP1-iLID-Halo

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAB061i

Plasmid

#79226

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4; PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-Cyto-TurboID

Plasmid

#149414

Purpose

Hepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

Cyto-TurboID

Use

AAV

Tags

V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro GFP siRNA

Plasmid

#12273

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

GFP siRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pMSCV LDTS-eLIR

Plasmid

#189000

Purpose

lipophagy-inducing adaptor protein

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-eLIR

Use

Retroviral

Tags

HA

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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