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BD2

Plasmid

#37486

Depositor

Linzhao Cheng

Article

Zou et al Blood. 2011 Oct 27;118(17):4599-608. Epu

Insert

loxP-PGK-Hyg-loxP

Use

Ipsc gene targeting

Promoter

PGK

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pN1-QUE2m

Plasmid

#129308

Purpose

QUEEN ATP sensor for 25degC

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-2m

Expression

Mammalian

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMito-GBPen

Plasmid

#128269

Purpose

Expression of mitochondrially targeted GBPen (GFP Nanobody)

Depositor

Stephen Royle

Article

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Insert

Mito-GBPen

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1

Plasmid

#187367

Purpose

Expresses human Plekhh1 labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5myc Rac1 N17

Plasmid

#15904

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Tags

myc

Expression

Mammalian

Mutation

T17N

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRK5myc Cdc42 wt

Plasmid

#15905

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Cdc42 (placental isoform) (CDC42 Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pWZL Blast ST

Plasmid

#13805

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

SV40 small T antigen (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pQE80_T5_Z18-ehaA_LacI

Plasmid

#186314

Purpose

Plasmid for inducible E coli expression of the Z18 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

Z18-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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HS-mKate2

Plasmid

#105945

Purpose

heat shock inducible transgenesis, cytosolic mKate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

mKate2

Expression

Bacterial

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-LacZshRNA

Plasmid

#73977

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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EZH2_N_FS_v2_Donor

Plasmid

#79901

Purpose

N-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR and TALEN

Tags

3xFLAG_Twin_Strep

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP(C1)-NIPP1

Plasmid

#44221

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

NIPP1 (PPP1R8 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pNB778

Plasmid

#60154

Purpose

Green click beetle luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Expression

Yeast

Promoter

LEU1pr

Available Since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pNB648

Plasmid

#60140

Purpose

NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Promoter

MET17pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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px330 VRER

Plasmid

#101729

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Rab27a cds in pGem-T Easy

Plasmid

#80546

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab27a (rab27a Zebrafish)

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pTCW007

Plasmid

#83525

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha1

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Venus-KRasCT

Plasmid

#99016

Purpose

Membrane marker

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Venus KRasCT

Expression

Mammalian

Promoter

CM

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMIG FLAG B56 gamma3

Plasmid

#13803

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 gamma 3 Flag (PPP2R5C Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pSG81-pLas-tetO-deGFPssrA

Plasmid

#102454

Purpose

Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Tags

ssrA

Expression

Bacterial

Promoter

pLas-tetO

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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