We narrowed to 23,585 results for: lenti

1. pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

   Plasmid

   #167200

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R D421N
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

   Plasmid

   #167198

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R E294K
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

   Plasmid

   #167197

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R M173I
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-3xFLAG-Puro PAK5 K478R/479R E144K

   Plasmid

   #167196

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R E144K
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG

   Plasmid

   #130678

   Purpose

   Expresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D409_F489del

   Promoter

   CMV

   Available Since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG

   Plasmid

   #130691

   Purpose

   Expresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
   

   Tags

   3xFLAG and 3xHA

   Expression

   Mammalian

   Mutation

   E653_G1960del

   Promoter

   CMV

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

   Plasmid

   #130690

   Purpose

   Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   1M_T1522delinsM, G1720_Y1846del

   Promoter

   CMV

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

   Plasmid

   #79380

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Chimera 2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

   Plasmid

   #79379

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Chimera 1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

    Plasmid

    #79382

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 4
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167935

    Purpose

    Inducible CRISPRi expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    KRAB-dCas9-DHFR
    

    Use

    Lentiviral

    Promoter

    tetON

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167937

    Purpose

    Inducible CRISPRa expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    dxCas9(3.7)-VPR
    

    Use

    Lentiviral

    Promoter

    tetON

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid

    #138148

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NES

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

    Plasmid

    #219537

    Purpose

    a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    NLS-tdMCP-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

    Plasmid

    #180273

    Purpose

    Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138150

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Mutation

    DR1 A>T

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

    Plasmid

    #207832

    Purpose

    telomere specific nuclease to create double-strand breaks at telomeres

    Depositor

    Roger Greenberg

    Article

    Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag, DD, ER, mCherry and FokI

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti UBC ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

    Plasmid

    #215012

    Purpose

    Express a ERphagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    KDEL
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE

    Plasmid

    #192193

    Purpose

    Lenti-BCMA-CAR-PRODH2

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)
    

    Use

    Lentiviral; Mammalian expression

    Mutation

    NA

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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