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We narrowed to 15,942 results for: LEA

Showing: 7101 - 7150 of 15942 results

  1. pEJS1350 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-92a CMV-BFP

    Plasmid
    #226109
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-92a Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226109-plasmid-map-sequence-id-448313

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  2. pEJS1351 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-378a CMV-BFP

    Plasmid
    #226110
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-378a Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226110-plasmid-map-sequence-id-448316

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  3. pEJS1353 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-33b CMV-BFP

    Plasmid
    #226112
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-33b Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226112-plasmid-map-sequence-id-448314

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  27. GNRHR-DuET

    Plasmid
    #213254
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GNRHR (GNRHR Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213254-plasmid-map-sequence-id-430804

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  28. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid
    #208977
    Purpose
    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208977-plasmid-map-sequence-id-416230

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  29. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

    Plasmid
    #208974
    Purpose
    Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    B103-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208974-plasmid-map-sequence-id-415835

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  30. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

    Plasmid
    #208971
    Purpose
    Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208971-plasmid-map-sequence-id-415826

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  31. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid
    #208970
    Purpose
    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208970-plasmid-map-sequence-id-415821

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  32. B-HeFSpCas9-A695Q

    Plasmid
    #207368
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A695Q
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    207368-plasmid-map-sequence-id-410902

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  33. pFSynW SYT9 D197, 199, 330, 332N IRES GFP

    Plasmid
    #195703
    Purpose
    Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter
    Depositor
    Edwin Chapman
    Article
    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.
    Insert
    Syt9 (Syt5 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    full-length mouse SYT9 with D197, 199, 330, 332N …
    Promoter
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195703-plasmid-map-sequence-id-383663

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  35. B-HeFSpCas9-A926Q

    Plasmid
    #207370
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A926Q
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207370-plasmid-map-sequence-id-410164

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  36. B-evoSpCas9-E526K

    Plasmid
    #207365
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-E526K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, Y515N, R661Q, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207365-plasmid-map-sequence-id-410163

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  37. B-evoSpCas9-V495M

    Plasmid
    #207363
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-V495M
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Y515N, K526E, R661Q, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207363-plasmid-map-sequence-id-410136

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  38. B-HeFSpCas9-A1060R

    Plasmid
    #207372
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A1060R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …
    Promoter
    CBh
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207372-plasmid-map-sequence-id-408921

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  39. B-HeFSpCas9-A1003K

    Plasmid
    #207371
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A1003K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …
    Promoter
    CBh
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207371-plasmid-map-sequence-id-408916

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  40. B-HeFSpCas9-A848K

    Plasmid
    #207369
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A848K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207369-plasmid-map-sequence-id-408749

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  41. B-HeFSpCas9-A661R

    Plasmid
    #207367
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A661R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207367-plasmid-map-sequence-id-408748

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  42. B-evoSpCas9-Q661R

    Plasmid
    #207366
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-Q661R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207366-plasmid-map-sequence-id-408747

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  43. B-evoSpCas9-N515Y

    Plasmid
    #207364
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-N515Y
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, K526E, R661Q, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207364-plasmid-map-sequence-id-408746

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  45. HypaR-SpCas9

    Plasmid
    #126757
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    Hypa2SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available Since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    126757-plasmid-map-sequence-id-412372

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  46. pCAGGS-SMS1-TEV-HAT

    Plasmid
    #202557
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS1 (SGMS1 Human)
    Use
    Tags
    Histidine-Affinity-tag (HAT) and TEV cleavable si…
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    202557-plasmid-map-sequence-id-399621

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  48. pRSF-G1TMSNKRS

    Plasmid
    #198323
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    198323-plasmid-map-sequence-id-393448

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Showing: 7101 - 7150 of 15942 results