We narrowed to 11,339 results for: GFP expression plasmids

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Pflp-18::NGL-1::spGFP11

Plasmid

#65828

Purpose

For trans-synaptic labeling

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

NLG-1 (nlg-1 Nematode)

Tags

spGFP11

Expression

Worm

Mutation

N142S

Promoter

Pflp-18

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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bdSUMO-GFP-HiBit

Plasmid

#170989

Purpose

For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Punc-4::NGL-1::spGFP1-10

Plasmid

#65827

Purpose

For trans-synaptic labeling

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

NLG-1 (nlg-1 Nematode)

Tags

spGFP1-10

Expression

Worm

Promoter

Punc-4

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-mGFP-pA(AV222)

Plasmid

#117858

Purpose

This plasmid expresses membrane anchored GFP to facility axon morphology study.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

mGFP

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-GFP

Plasmid

#127537

Purpose

Expresses human SOX2 and eGFP via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-IRAlus-Lin28

Plasmid

#92347

Purpose

Plasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Lin28 gene derived IR Alu element containing 3'UTR (LIN28A Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alu-Lin28

Plasmid

#92346

Purpose

Plasmid for expression of GFP gene with a Lin28 gene derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Lin28 gene derived Alu element containing 3'UTR (LIN28A Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_mCherry-CD8-vhhGFP

Plasmid

#163930

Purpose

Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-R9-GFP-HiBit

Plasmid

#170990

Purpose

For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-R9-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1-EGFP

Plasmid

#112857

Purpose

plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

Plasmid

#48253

Purpose

LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

CRY2PHR-NLS-VP64

Use

AAV; Tale

Tags

2A_GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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AV4-EF1-GFP

Plasmid

#124971

Purpose

Control for AV4-EF1-NMNAT2

Depositor

Kazuhiro Oka

Article

Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

Insert

GFP

Use

AAV

Promoter

EF1-alpha

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-MIOX only

Plasmid

#166683

Purpose

Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

GFP-MIOX

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL10

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pAT15516_BEAR-GFP-2in1

Plasmid

#174082

Purpose

BEAR-GFP plasmid with a gRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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PB-Aka-P2A-GFP

Plasmid

#120867

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc

Expression

Mammalian

Mutation

insert Akaluc and a P2A site

Promoter

CAG Promoter

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT_GFP 150TAG

Plasmid

#140016

Purpose

Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG, K46E (Please see depositor comments below)

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ cMyc-oDam_f_GFPoNLS

Plasmid

#85818

Purpose

iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

cMyc oDam_f_GFPoNLS

Tags

cMyc and oNLS (optimized nuclear localization sig…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-CAAX

Plasmid

#86056

Purpose

A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Homo sapiens Harvey rat sarcoma (HRAS Human)

Tags

GFP

Expression

Mammalian

Mutation

contains the region from 825-857 of HRAS as a far…

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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PB533_2E12LI-sfGFP

Plasmid

#167527

Purpose

H3K27me3-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Tjalsma et al EMBO Rep. 2021 Feb 19:e51989. doi: 10.15

Insert

2E12LI-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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