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pCAG-mWasabi-ER

Plasmid

#164165

Purpose

Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-Amida

Plasmid

#15362

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TCF3 (E2A) fusion partner (TFPT Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pMpGWB310

Plasmid

#68638

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ496

Plasmid

#44488

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

500bp Mos1, unc-119, fosmid cassette

Use

Fosmid recombineering cassette

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pRL1124

Plasmid

#70690

Purpose

provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Inserts

avaiM
eco47iiM
ecot22iM

Use

Bacterial shuttle vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_5_neg_Control

Plasmid

#130558

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mTFP1-VSVG-16

Plasmid

#55517

Purpose

Localization: Golgi/ER Transport, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

VSVG

Tags

mTFP1

Expression

Mammalian

Mutation

deltaK VSVG ts045

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
BAT-lux

Plasmid

#20890

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7xTCF binding sites, Siamois minimal promoter

Use

Luciferase

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements fused upstrea…

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pColE1_sgRNA_M7NS_AmpR

Plasmid

#119157

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M2S_AmpR

Plasmid

#119154

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBRC169

Plasmid

#79670

Purpose

DsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

DsRED-monomer (169–225)/RC169

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB307

Plasmid

#68635

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLBC0060

Plasmid

#172783

Purpose

Level 0 module containing NT2_cYFP3XHA

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238) 3XHA tag

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0061

Plasmid

#172784

Purpose

Level 0 module containing CT_cYFP3XHA

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238) 3XHA tag

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas7/pHMGWA

Plasmid

#81184

Purpose

Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-FOXI2

Plasmid

#153127

Purpose

Expression of Flag tagged human FOXI2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXI2 (FOXI2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ66

Plasmid

#24981

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2010 Jun;7(6):451-3. doi: 1

Insert

Cbr-unc-119 Punc-122::GFP

Use

Multisite gateway

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-mEYFP

Plasmid

#87666

Purpose

retroviral vector encoding cre dependent expression of membrane EYFP

Depositor

Lynette Lim, Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-EYFP

Use

Retroviral

Tags

palmitylation

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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LC3-mEYFP

Plasmid

#182865

Purpose

Autophagy Marker

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

rat LC3 (Anxa3 Rat)

Expression

Mammalian

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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