We narrowed to 7,647 results for: Ski;

1. pmCellFree_KA1_ZIKV NS1

   Plasmid

   #145342

   Purpose

   Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term eGFP

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS1
   

   Use

   Tags

   8xHis and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCellFree_G06_ZIKV NS1

   Plasmid

   #145352

   Purpose

   Cell-free expression vector for Zika virus NS1 protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS1
   

   Use

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNRB143

   Plasmid

   #36452

   Depositor

   Douglas Bishop

   Article

   Bishop et al Genes Cells. 1999 Aug;4(8):425-44.

   Insert

   RAD54 (RAD54 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   T7

   Available Since

   Aug. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pRL1124

   Plasmid

   #70690

   Purpose

   provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.

   Depositor

   Peter Wolk

   Article

   Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

   Inserts

   avaiM
   eco47iiM
   ecot22iM
   

   Use

   Bacterial shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NODALvar-CAG-AAVS1

   Plasmid

   #115262

   Purpose

   For stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cells

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL (NODAL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pALPS_3'LTR GFP@gag start SFFV-

   Plasmid

   #101337

   Purpose

   Repaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmCellFree_KA2_ZIKV NS4A

   Plasmid

   #145366

   Purpose

   Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS4A
   

   Use

   Tags

   8xHis and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SEPT5 Mut(S161A/S327A)

   Plasmid

   #27275

   Depositor

   Harish Pant

   Article

   Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

   Insert

   Septin 5 (SEPTIN5 Human)
   

   Use

   Tags

   Myc (MASMQKLISEEDL)

   Expression

   Mammalian

   Mutation

   S161A and S327A

   Promoter

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3.0_mitoNanobody IRES EGFP

   Plasmid

   #136621

   Purpose

   Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   NTOM20-SNAP-Tag-EnhancerNb (IRES EGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCSN074

    Plasmid

    #166731

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET51b(+)_LAMA-N95 (for GFP)

    Plasmid

    #130716

    Purpose

    Plasmid encoding the bacterial expression of LAMA-N95 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-N95
    

    Use

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ptagBFP-LacI-NLS-VP16

    Plasmid

    #103837

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-LacI-NLS-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA5/FRT-ChemoB-NAD

    Plasmid

    #193824

    Purpose

    CMV overexpression of ChemoB-NAD in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EBFP2:N39Y, A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pALPS_3'LTR GFP@gag start SFFV rev

    Plasmid

    #101339

    Purpose

    LTR drives GFP and internal SFFV promoter drives codon optimized rev

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP, rev
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUASP YFP Rab4 DN

    Plasmid

    #37691

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab4 (Rab4 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    S22N (dominant negative)

    Promoter

    GAL4

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pmCellFree_KA1_ZIKV PrM

    Plasmid

    #145340

    Purpose

    Cell-free/mammalian expression vector for Zika virus PrM protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV PrM
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available Since

    April 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmCellFree_KA1_ZIKV NS3

    Plasmid

    #145345

    Purpose

    Cell-free/ mammalian expression vector for Zika virus NS3 protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS3
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available Since

    April 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmCellFree_KA1_ZIKV NS4A

    Plasmid

    #145346

    Purpose

    Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS4A
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available Since

    April 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS NL4-3 envFS eGFP Δgag/pol

    Plasmid

    #101343

    Purpose

    Deletion from the start of gag until 229 bases before the cPPT

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta GagPol

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiCRISPR V2 sgACO1_1

    Plasmid

    #169885

    Purpose

    Disrupt ACO1

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting ACO1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge