We narrowed to 15,942 results for: LEA

1. pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP

   Plasmid

   #196876

   Purpose

   Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   LynEGFP
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

   Plasmid

   #196883

   Purpose

   Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-ZsGreen-bGH
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

   Plasmid

   #196884

   Purpose

   Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-Lyn-GFP-bGH
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]

   Plasmid

   #196886

   Purpose

   Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-ZsGreen-bGH
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBT9-FTS

   Plasmid

   #198526

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FTS (AKTIP Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBT9-FHIP-L

   Plasmid

   #198528

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FHIP-L (FHIP1A Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBridge-tyrosinase.σ3A

   Plasmid

   #197513

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-3 σ3A
   

   Use

   Tags

   GAL4-DNA binding domain fragment, HA tag, and nuc…

   Expression

   Yeast

   Mutation

   silent substitution in codon 2 of tyrosinase tail…

   Promoter

   ADH1 and MET25

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-DmPAN2-del1047-1063-GSG-dsRNAres_Y

   Plasmid

   #148070

   Purpose

   Insect Expression of DmPAN2-del1047-1063-GSG-dsRNAres

   Depositor

   Elisa Izaurralde

   Article

   Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

   Insert

   DmPAN2-del1047-1063-GSG-dsRNAres (PAN2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation compared the the sequence gei…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7-EGFP-C1-HsNot1iso1-4x mut_R

   Plasmid

   #147371

   Purpose

   Mammalian Expression of HsNot1iso1-4xmut

   Depositor

   Elisa Izaurralde

   Article

   Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

   Insert

   HsNot1iso1-4xmut (CNOT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A deletion of 5 AA 822-827 SKMKPS-> T and one …

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-EGFP-C1-HsNot1iso1-K1208AH1212AK1218A_R

    Plasmid

    #147372

    Purpose

    Mammalian Expression of HsNot1iso1-K1208AH1212AK1218A

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-K1208AH1212AK1218A (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7-EGFP-C1-HsNot1iso1-K1208EH1212TK1218E_R

    Plasmid

    #147373

    Purpose

    Mammalian Expression of HsNot1iso1-K1208EH1212TK1218E

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-K1208EH1212TK1218E (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7-EGFP-C1-HsNot1iso1-P1209YP1257Y_R

    Plasmid

    #147374

    Purpose

    Mammalian Expression of HsNot1iso1-P1209YP1257Y

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-P1209YP1257Y (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT7-EGFP-C1-HsNot1iso1-P1257Y_R

    Plasmid

    #147375

    Purpose

    Mammalian Expression of HsNot1iso1-P1257Y

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-P1257Y (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-DmNot1-K1277A-dsRNAres_R

    Plasmid

    #147359

    Purpose

    Insect Expression of DmNot1-K1277A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmNot1-K1277A-dsRNAres (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    10 silent mutations and two non silent N605S, K75…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmNot1-Q1271A-dsRNAres_R

    Plasmid

    #147360

    Purpose

    Insect Expression of DmNot1-Q1271A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmNot1-Q1271A-dsRNAres (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    10 silent mutations and two non silent N605S, K75…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmNot1-R1267A-dsRNAres_R

    Plasmid

    #147361

    Purpose

    Insect Expression of DmNot1-R1267A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmNot1-R1267A-dsRNAres (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    10 silent mutations and two non silent N605S, K75…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLNHA-C1-HsNot1iso1_1-1089_K

    Plasmid

    #146804

    Purpose

    Mammalian Expression of HsNot1iso1_1-1089

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1_1-1089 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of 5 AA compared to the sequence given b…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLNHA-C1-HsNot1iso1_1085-1605_K

    Plasmid

    #146805

    Purpose

    Mammalian Expression of HsNot1iso1_1085-1605

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1_1085-1605 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-EGFP-C1-HsNot1iso1_1-1089_K

    Plasmid

    #146806

    Purpose

    Mammalian Expression of HsNot1iso1_1-1089

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1_1-1089 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of 5 AA compared to the sequence given b…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-EGFP-C1-HsNot1iso1_1085-1605_K

    Plasmid

    #146807

    Purpose

    Mammalian Expression of HsNot1iso1_1085-1605

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1_1085-1605 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1 silent mutation compared to the sequence of iso…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAc5.1B-lambdaN-DmPABPC1-RRM1-RRM2-V5His6_H

    Plasmid

    #146490

    Purpose

    Insect Expression of DmPABPC1-RRM1-RRM2

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmPABPC1-RRM1-RRM2 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAc5.1B-lambdaN-DmPABPC1-RRM2-RRM3-V5His6_H

    Plasmid

    #146491

    Purpose

    Insect Expression of DmPABPC1-RRM2-RRM3

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmPABPC1-RRM2-RRM3 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAc5.1B-lambdaN-DmPABPC1-RRM3-RRM4-V5His6_H

    Plasmid

    #146492

    Purpose

    Insect Expression of DmPABPC1-RRM3-RRM4

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmPABPC1-RRM3-RRM4 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAc5.1B-lambdaN-HA-DmGW182-V5His6_H

    Plasmid

    #146499

    Purpose

    Insect Expression of DmGW182

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmGW182 (gw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAc5.1B-DmPABPC1-RRM1-RRM2-V5His6_H

    Plasmid

    #146479

    Purpose

    Insect Expression of DmPABPC1-RRM1-RRM2

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmPABPC1-RRM1-RRM2 (pAbp Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEST15-Eps8L2

    Plasmid

    #196534

    Purpose

    Expression of GST-Eps8L2-FL

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2 (EPS8L2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter; lac UV5 promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRK5mycGW-Eps8L2_529-715

    Plasmid

    #196543

    Purpose

    Expression of myc-Eps8L2_529-715

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2_529-715 (EPS8L2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRK5mycGW-Eps8L2

    Plasmid

    #196542

    Purpose

    Expression of myc-Eps8L2-FL

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2 (EPS8L2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mKate2-DrCav1 in pT3TS-Dest

    Plasmid

    #194293

    Purpose

    In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    caveolin (cav1.S Frog)
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p3E-DrCavin1a

    Plasmid

    #194291

    Purpose

    Multisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXM

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    cavin1a (cavin1a Zebrafish)
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pX459-dTLN1 exon

    Plasmid

    #176033

    Purpose

    A vector for the CRISPR-Cas9 system targeting an exon of dog TLN1 (talin1)

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pX459-dTLN1 intron

    Plasmid

    #176224

    Purpose

    A vector for the CRISPR-Cas9 system targeting an intron of dog TLN1 (talin1)

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

    Plasmid

    #182126

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

    Plasmid

    #182121

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

    Plasmid

    #182124

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLKO_AU1.C.NWS_mCherry-NLS

    Plasmid

    #178282

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.NWS and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HIS_TEV_hsSYX3

    Plasmid

    #179290

    Purpose

    E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    residues 393-792 from accession number NP_0010361…

    Promoter

    T7lac

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHcRed-NPMMLF1nes-C1

    Plasmid

    #131849

    Purpose

    Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    This is a control deletion contruct of the cytop…

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-α-COPI-WD40-Arg57Ala

    Plasmid

    #180759

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Arg57Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Arg57Ala, Leu1…

    Promoter

    CMV

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1-α-COPI-WD40-Tyr139Ala

    Plasmid

    #180761

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Tyr139Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Tyr139Ala, Leu…

    Promoter

    CMV

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1-α-COPI-WD40-Asp115Ala

    Plasmid

    #180760

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Asp115Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Asp115Ala, Leu…

    Promoter

    CMV

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLV V5-Chpt1

    Plasmid

    #175155

    Purpose

    Lentiviral expression of V5-tagged mouse Chpt1

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Chpt1 (Chpt1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLKO_NWS.S.V5_mCherry-NLS

    Plasmid

    #178281

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    NWS.S.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLKO_NWS.Ollas.V5_mCherry-NLS

    Plasmid

    #178280

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    NWS.Ollas.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLKO_HSV.NWS.V5_mCherry-NLS

    Plasmid

    #178275

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.NWS.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLKO_HSV.NWS.VSVg_mCherry-NLS

    Plasmid

    #178274

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.NWS.VSVg and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLKO_HSV.NWS.Ollas_mCherry-NLS

    Plasmid

    #178272

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HSV.NWS.Ollas and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLKO_HA.VSVg.V5_mCherry-NLS

    Plasmid

    #178271

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.VSVg.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLKO_HA.S.V5_mCherry-NLS

    Plasmid

    #178270

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.S.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLKO_HA.Ollas.V5_mCherry-NLS

    Plasmid

    #178268

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.Ollas.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge