We narrowed to 27,367 results for: STI

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pAV0605

Plasmid

#133503

Purpose

Expression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-RitC-terminator(tdh1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0746

Plasmid

#133480

Purpose

Expression in fission yeast from promoter prga3 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(rga3)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSW377 - pTMO5

Plasmid

#115988

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pTMO5

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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DeltaNIRD C95S KVS-1

Plasmid

#113068

Purpose

Expresses worm truncated KVS1.2 C113S in mammalian cells

Depositor

Federico Sesti

Article

Cai et al Nat Neurosci. 2009 May;12(5):611-7. doi:

Insert

C113S K+ voltage sensitive channel subunit 1.2 (kvs-1 Nematode)

Expression

Mammalian

Mutation

C113S+first 40 AA deletion

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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CTL2 (M2225R)

Plasmid

#96952

Purpose

Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

Depositor

Douglas Rees

Article

Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

Insert

CTL2 (M222R) of PIEZO1

Tags

6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

Plasmid

#91945

Purpose

Bacterial expression of maize SAE1 and truncated SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Mutation

Truncation of ZmSAE2 to mimic a splice variant

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S46X with 5'UTR

Plasmid

#87729

Purpose

Expresses S46X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S46X

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S44L with 5'UTR

Plasmid

#87727

Purpose

Expresses S44L spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S44L

Promoter

pCMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/E112K with 5'UTR

Plasmid

#87723

Purpose

Expresses E112K spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

E112K

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/L195V with 5'UTR

Plasmid

#87725

Purpose

Expresses L195V spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

L195V

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3A/myc-M1E112K

Plasmid

#87715

Purpose

Expresses myc-M1E112K spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; E112K

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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const #7-RFP

Plasmid

#81081

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23109

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #6-RFP

Plasmid

#81080

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23117

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1 N297A

Plasmid

#66723

Purpose

Constitutive expression of FPR1 N297A in mammalian cells.

Depositor

Heini Miettinen

Article

Gripentrog et al Cell Signal. 2008 Feb;20(2):424-31. Epub

Insert

FPR1 (haplotype 3) (FPR1 Human)

Expression

Mammalian

Mutation

N297A

Promoter

SR-alpha

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-hPREP

Plasmid

#179387

Purpose

Express human prolyl oligopeptidase (tagged) in bacterial cells

Depositor

Christian Gruber

Article

Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

Insert

human prolyl oligopeptidase (PREP Human)

Tags

GST

Expression

Bacterial

Promoter

TAC

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCnRed

Plasmid

#232908

Purpose

Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

gam (gam phage Lambda, Synthetic)

Use

Synthetic Biology

Promoter

Pbad-rha

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPRi

Plasmid

#199808

Purpose

vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

Depositor

Steve Salipante

Article

Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

Inserts

dCas9
sgRNA with GFP cassette

Use

CRISPR

Promoter

P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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hKv2.1-pEGFP-N1

Plasmid

#111532

Purpose

Expresses human KCNB1 in mammalian cells for imaging

Depositor

Federico Sesti

Article

Yu et al Cell Death Dis. 2017 Apr 6;8(4):e2737. d

Insert

human Kv2.1 (KCNB1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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