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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 2861 - 2880 of 11938 results

  2. pGS1021

    Plasmid
    #59575
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Publication
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Promoter
    None
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    59575-plasmid-map-sequence-id-92981

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  3. pGS1022

    Plasmid
    #59576
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Publication
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Promoter
    None
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    59576-plasmid-map-sequence-id-92983

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  4. pEMS1229

    Plasmid
    #29115
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple203 (SLC7A5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Oct. 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29115-plasmid-map-sequence-id-13530

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  6. pOPS0314

    Plasmid
    #115507
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB
    Depositor
    Philip Poole
    Publication
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115507-plasmid-map-sequence-id-224284

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  8. pOPS0359

    Plasmid
    #115508
    Purpose
    Reporter Plasmid. Taurine-inducible expression of GFP
    Depositor
    Philip Poole
    Publication
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    sfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Taurine-inducible promoter of S. meliloti Promote…
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115508-plasmid-map-sequence-id-224277

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  11. pcDNA_RGG-GFP-RGG_withTEVsite

    Plasmid
    #124939
    Purpose
    Expresion of RGG_GFP-RGG
    Depositor
    Matthew Good, Daniel Hammer, Benjamin Schuster
    Publication
    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    TEV site between RGG and EGFP
    Available since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124939-plasmid-map-sequence-id-247690

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  12. ORAI1-YFP V107M

    Plasmid
    #114180
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).
    Depositor
    Nicolas Demaurex
    Publication
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ORAI1-YFP V107M (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    V107M
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114180-plasmid-map-sequence-id-218674

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  14. mEos3-Rac1

    Plasmid
    #92039
    Purpose
    Expresses mEos3-Rac1 fusion protein
    Depositor
    Ji Yu
    Publication
    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAC1 (RAC1 Human)
    Tags
    mEos3
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    CMV
    Available since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92039-plasmid-map-sequence-id-180748

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  16. mEos3.2GPI

    Plasmid
    #203759
    Purpose
    Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Publication
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD58 c-term (CD58 Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203759-plasmid-map-sequence-id-404788

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  17. PMmEos3.2

    Plasmid
    #203755
    Purpose
    Encodes the membrane anchor of Lyn including the first 10 residues of the N terminus, with 1 myristoylation and 1 palmitoylation, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Publication
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn n-term (LYN Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203755-plasmid-map-sequence-id-403338

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Showing: 2861 - 2880 of 11938 results