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pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpA

Plasmid

#87580

Purpose

PV1 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

Neomycin Resistance Gene

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW807_pCAG-PV4-loxP-mCherry-BGHpA

Plasmid

#87583

Purpose

PV4 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW770_pCAG-PV4-loxP-mRuby2-BGHpA

Plasmid

#87577

Purpose

PV4 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mRuby2

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW707_pCAG-PV3-FRT-loxP-mCherry

Plasmid

#87571

Purpose

PV3 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBW705_pCAG-PV1-FRT-lox2272-STOP

Plasmid

#87569

Purpose

PV1 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

Neomycin Resistance Gene

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW706_pCAG-PV2-lox2272-GFP

Plasmid

#87570

Purpose

PV2 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mCBFKOgRNA5

Plasmid

#84366

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
mCBFKOgRNA2

Plasmid

#84365

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-myc-mCE

Plasmid

#82470

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Capping Enzyme (Rngtt Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDS2-aurZ

Plasmid

#70151

Purpose

Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

aurZ

Expression

Yeast

Promoter

Tef

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDS3-aurJ

Plasmid

#70152

Purpose

Fusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

aurJ

Expression

Yeast

Promoter

GPD1

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only
pDS2

Plasmid

#70135

Purpose

CEN/ARS yeast shuttle vector with DS2 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

DS2

Use

Synthetic Biology

Promoter

AgTEF1

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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PU.1-delta75-100 LZRS

Plasmid

#44634

Depositor

Ellen Rothenberg

Article

Dionne et al Dev Biol. 2005 Apr 15;280(2):448-66.

Insert

PU.1 Δ75-100 (Spi1 Mouse)

Use

Retroviral

Tags

EGFP and IRES

Expression

Mammalian

Mutation

deletion of aa 75-100

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
pTOPO-rRNA-5'ETS 923 to 1179

Plasmid

#23995

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rRNA 5'ETS ntds 923 to 1179

Use

Bacterial cloning - topo

Mutation

None

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTOPO-rRNA-5'ETS 1 to 177

Plasmid

#23993

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rDNA 5'ETS ntds 1 to 177

Use

Bacterial cloning - topo

Mutation

None

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTOPO-25S rDNA

Plasmid

#23996

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

25S rRNA from +5270 to oligo y

Use

Bacterial cloning - topo

Mutation

none

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pSL5 SMR1713

Plasmid

#28028

Depositor

Susan Rosenberg

Article

Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

Insert

MutL (mutL E. coli)

Expression

Bacterial

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
Sgf73(1-106)/PET32a

Plasmid

#26962

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Sgf73(1-106) (SGF73 Budding Yeast)

Tags

His6 and Trx

Expression

Bacterial

Mutation

with TEV site to cleave the N-terminal his+Trx tag

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pDL11c

Plasmid

#17833

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, argB_1626,

Use

Synthetic Biology; Aspergillus nidulans expression

Mutation

The argB_1626 mutation consists of two sequential…

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pA9HD

Plasmid

#8588

Depositor

Cynthia Wolberger

Article

LaRonde-LeBlanc et al Genes Dev 2003 Aug 15;17(16):2060-72.

Insert

HoxA9 190-271with hexapeptide (HOXA9 Human)

Tags

His

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only