We narrowed to 7,094 results for: plasmid dna
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Plasmid#45978PurposeExpresses yeast Flp driven by the constitutive promoter pE from phage P2DepositorInsertflp
UseSynthetic BiologyExpressionBacterialMutationflp driven by the constitutive promoter pE from p…PromoterpE (from phage P2)Available SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mAIRN HO
Plasmid#207411PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertmAIRN
ExpressionBacterial and MammalianAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAIRN CD
Plasmid#207412PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertmAIRN
ExpressionBacterial and MammalianAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIG3 (pCMV-IRES-GFP version 3)
Plasmid#78264PurposeLentiviral empty cloning vector containing IRES and GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsIRES-EGFPExpressionMammalianPromoterCMV, lentiviral LTRAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
ECFP HO
Plasmid#207413PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertECFP
ExpressionBacterial and MammalianAvailable SinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
ECFP CD
Plasmid#207414PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertECFP
ExpressionBacterial and MammalianAvailable SinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-shRNA-Tet1
Plasmid#85742PurposeshRNA against mouse Tet1DepositorInsertshRNA Tet1
UseAAVTagsEYFPAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHJ4: LexA-VHH-Caffeine
Plasmid#108242PurposeLexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.DepositorInsertLexA DNA binding domain fusing with VHH-Caffeine antibody
UseSynthetic BiologyPromoterpLacO1Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
DR274
Plasmid#42250Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcriptionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR; Zebrafish expressionExpressionWormPromoterT7Available SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAP118
Plasmid#105495PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusionsDepositorInsertHupA-SmBiT/HupA-LgBiT
UseAtc-dependent expression in c. difficilePromoterPtetAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 WT
Plasmid#78260PurposepsiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertfully complimentary let-7 target site
UseLuciferase; Microrna activityExpressionMammalianAvailable SinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-greenlantern
Plasmid#202141PurposeLevel 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertGreen lantern gene
UseSynthetic BiologyMutationNoneAvailable SinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSpCas9n(BB) (PX460)
Plasmid#48873PurposeCas9n (D10A nickase mutant) from S. pyogenesDepositorHas ServiceCloning Grade DNAInserthSpCas9n
UseCRISPRTags3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMax_mPlum
Plasmid#177830PurposeHost Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)DepositorInsertmPlum
ExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AY22_pgRNA.R5.1
Plasmid#100294PurposeCCR5-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6 RNA Pol III promoter human snRNAAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLP-mScarlet-I
Plasmid#209993PurposeContains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmidsDepositorInsertmScarlet-I
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GADD45 WT-luc
Plasmid#8356DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag-P2A-tagBFP
Plasmid#174141PurposeExpresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.DepositorInsertdCas9, 10xSunTag, tagBFP
TagsHA and tagBFPExpressionMammalianPromoterSV40Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor
Plasmid#229787PurposeBxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)DepositorInsertLacZ; ZF10-NS3-p65
UseSynthetic BiologyExpressionMammalianPromoterCAGAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only