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pTYB3-human PUM1-HD MUT7-2 (S1079N-E1083Q)-intein-CBD

Plasmid

#73299

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

S1079N-E1083Q

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pES007 (pTet-qacR 5/pQacA-deGFP)

Plasmid

#69036

Purpose

reporter with qacR 5 on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Mutation

W61Y, E90Q, F102S, M116Q, Y119L, E120Q

Promoter

pQacA and pTet

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A-miR-375-mutC&D

Plasmid

#53712

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site C&D (refer t…

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutE

Plasmid

#53710

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site E (refer to cita…

Available Since

July 11, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA&B

Plasmid

#53711

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site A&B (refer t…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutD

Plasmid

#53709

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site D (refer to cita…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutC

Plasmid

#53708

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site C (refer to cita…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutB

Plasmid

#53707

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site B (refer to cita…

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA

Plasmid

#53706

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site A (refer to cita…

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pFB-EF1a-DIO-Rpl22l1-myc-Flag-2A-tdTomato-WPRE-hGHpA

Plasmid

#246243

Purpose

Cre dependent flag-tagged Rpl22l1.

Depositor

D. James Surmeier

Article

Tanimura et al Neuron. 2019 Feb 6;101(3):444-458.e6. do

Insert

Rpl22l1 (Rpl22l1 Mouse)

Use

AAV

Tags

Myc-FLAG-T2A-tdTomato

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Bsd-CMV>hCDK11B

Plasmid

#239211

Purpose

Expresses CDK11B in mammalian cell lines

Depositor

Jason Sheltzer

Article

Julian et al bioRxiv 2025.08.03.668359; doi: https://

Insert

cyclin dependent kinase 11B (CDK11B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-SoxN-A22-EGFP

Plasmid

#181731

Purpose

Galactose inducible expression of SoxN-A22-EGFP

Depositor

Alejandro Chavez

Insert

SoxN-A22-EGFP (SoxN Fly)

Tags

3xFLAG-EGFP

Expression

Yeast

Mutation

extended repeat of 22 Ala residues replacing nati…

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro -GFP-I77P I78P REX1-IRES-mCherry

Plasmid

#232245

Purpose

Mammalian expression of I77P I78P REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

I77P I78P REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Tags

AcGFP1

Expression

Mammalian

Mutation

I77P I78P

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

Plasmid

#209787

Purpose

Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

TNT4, TadA8e, SpRY N, inteinN, miR122 TS

Use

AAV

Promoter

TNT4

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Plasmid

#219653

Purpose

General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus

Depositor

Thomas Oertner

Insert

2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Use

AAV

Tags

2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)

Expression

Mammalian

Promoter

nEf Addgene # 149296

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (D62Q)

Plasmid

#200979

Purpose

Mammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain; D62Q mutation fo…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EFNB2 (D62Q)

Plasmid

#200976

Purpose

Mammalian expression plasmid for myc-tagged EFNB2 (D62Q specificity mutant)

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

D62Q (increases specificity towards henipaviral G…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam_AG

Plasmid

#148831

Purpose

Bacterial Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Bacterial

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBam_AA

Plasmid

#148287

Purpose

Insect Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam_AA

Plasmid

#148289

Purpose

Insect Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-DmBam_AG

Plasmid

#148837

Purpose

Mammalian Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Mammalian

Mutation

one non silent mutation A239 to S, described as …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-DmBam_AG

Plasmid

#148840

Purpose

Mammalian Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Mammalian

Mutation

one non silent mutation A239 to S, described as …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-YE2

Plasmid

#154146

Purpose

Expresses dLbCpf1-BE-YE2(dLbCas12a-BE-YE2) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YE2 (dCas12a-BE-YE2)

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R132E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGG431

Plasmid

#165615

Purpose

Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Corrected homology relative to wild type SpCas9 (…

Promoter

TEF1

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

Plasmid

#160625

Purpose

GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

U6-26-1gRNA-DFR 2.1

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

U6-26

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Drosophila Nedd2-like caspase (DRONC)

Plasmid

#157778

Purpose

E. coli expression vector pET23 with C-term 6xHis tag

Depositor

Guy Salvesen

Article

Snipas et al Cell Death Differ. 2008 May;15(5):938-45

Insert

Drosophila Nedd2-like caspase (DRONC) (Dronc Fly)

Tags

6x HIS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

Plasmid

#105680

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFLAG TPP1 88-249 delta166-172

Plasmid

#53550

Purpose

mammalian expression of TPP1 OB-fold domain with 166-172 replaced with "GSSG"

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

contains aa 88-249, with aa 166-172 replaced with…

Promoter

CMV

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

Plasmid

#15602

Depositor

Susan Lindquist

Article

Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Insert

Sup35 NM (SUP35 Candida albicans, Budding Yeast)

Tags

7xHis and Cysteine

Expression

Bacterial

Mutation

4 G/A. Four glycines mutated to alanines. The pos…

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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