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Showing: 4321 - 4350 of 10852 results

  8. Dual-sgRNA_hU6-mU6

    Plasmid
    #154194
    Purpose
    Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154194-plasmid-map-sequence-id-308648

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  11. Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

    Plasmid
    #216125
    Purpose
    Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.
    Depositor
    Stefan Kubicek
    Article
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Insert
    sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216125-plasmid-map-sequence-id-428733

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  12. CMV-ER-LAR-GECO1

    Plasmid
    #61244
    Purpose
    Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells
    Depositor
    Robert Campbell
    Article
    Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi
    Insert
    LAR-GECO1
    Use
    Tags
    ER-retention sequence: KDEL and ER-targeting sequ…
    Expression
    Mammalian
    Mutation
    Substitutions relative to R-GECO1: V51W/I113V/N35…
    Promoter
    CMV
    Available since
    March 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61244-plasmid-map-sequence-id-117077

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  28. Plenti-ARE-HSVTK-luc

    Plasmid
    #161789
    Purpose
    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers
    Depositor
    Hans-Guido Wendel
    Article
    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi
    Inserts
    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and Murine Gclc ARE
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161789-plasmid-map-sequence-id-324302

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  29. pCDF-Frb-v1

    Plasmid
    #201923
    Purpose
    E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.
    Depositor
    Ryan Mehl, Richard Cooley
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    FrbA
    FrbB
    FrbC
    FrbD
    FrbE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (modified), T7 (modified, moderate), T7 (modif…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201923-plasmid-map-sequence-id-395412

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Showing: 4321 - 4350 of 10852 results