We narrowed to 14,259 results for: LEA

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mNeonGreen-Smad3

Plasmid

#172098

Purpose

Expresses fluorescently tagged human Smad3 in mammalian cells.

Depositor

Lea Goentoro

Publication

Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

Smad 3 (Smad3 Mouse)

Tags

mNeonGreen

Expression

Mammalian

Mutation

C49S mutation in mCerulean

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER-scramble

Plasmid

#118349

Purpose

This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

Depositor

Lea Sistonen

Publication

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

scrambled shRNA

Expression

Mammalian

Mutation

WT

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSUPER-hHSF1 shRNA

Plasmid

#118345

Purpose

This plasmid expresses shRNA against hHSF1.

Depositor

Lea Sistonen

Publication

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF1 shRNA (HSF1 Human)

Expression

Mammalian

Mutation

WT

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-NSD1 [N312/10] (Recombinant Antibody)

Antibody

#192041-rAb

Purpose

Anti-NSD1 (Human) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended applications

Immunocytochemistry

Reactivity

Human

Source species

Mouse

Isotype

IgG2a

Trial size

Available to purchase

Available since

March 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits
hHSF1 K80Q

Plasmid

#118351

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

Depositor

Lea Sistonen

Publication

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80Q

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1 WT

Plasmid

#71726

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

Depositor

Lea Sistonen

Publication

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 VP16 AD (HSF1 Herpes Simplex Virus protein VP16, Human, Budding Yeast)

Expression

Mammalian

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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hHSF4b WT

Plasmid

#118356

Purpose

This plasmid expresses human HSF4b WT.

Depositor

Lea Sistonen

Publication

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Expression

Mammalian

Mutation

WT

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hHSF1 S303/7A

Plasmid

#118365

Purpose

This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Publication

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A and S307A

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hHSF1 K80R

Plasmid

#118350

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to R.

Depositor

Lea Sistonen

Publication

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80R

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hHSF1 K298R

Plasmid

#118362

Purpose

This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

Depositor

Lea Sistonen

Publication

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K298R

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hSUMO1-HIS

Plasmid

#118361

Purpose

This plasmid expresses the full length human SUMO1.

Depositor

Lea Sistonen

Publication

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hSUMO1 (SUMO1 Human)

Tags

HIS

Expression

Mammalian

Mutation

Please see Depositor Comments

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hHSF1 RNAi resistant

Plasmid

#118347

Purpose

This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

Depositor

Lea Sistonen

Publication

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

RNAi resistance mutations (does not change amino …

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hGATA-1 K137R

Plasmid

#118353

Purpose

This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

Depositor

Lea Sistonen

Publication

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K137R

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEQ111m

Plasmid

#40190

Depositor

Paul Modrich

Publication

Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

Insert

EcoRI endonuclease & EcoRI methylase

Expression

Bacterial

Mutation

changed Glutamic Acid 111 to Glutamine

Promoter

Lambda PL

Available since

Nov. 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Anti-NALCN [N185/7R] (Recombinant Antibody)

Antibody

#190893-rAb

Purpose

Anti-NALCN (Rat) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended applications

Immunohistochemistry and Western Blot

Reactivity

Mouse and Rat

Source species

Mouse

Isotype

IgG2a

Trial size

Available to purchase

Available since

Dec. 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits
pEQ111m

Plasmid

#40190

Depositor

Paul Modrich

Publication

Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-2

Insert

EcoRI endonuclease & EcoRI methylase

Expression

Bacterial

Mutation

changed Glutamic Acid 111 to Glutamine

Promoter

Lambda PL

Available since

Nov. 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3-HA-TRAIL-R1

Plasmid

#61382

Purpose

Expresses HA tagged TRAIL receptor 1

Depositor

Francis Chan

Publication

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 1

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R1 leader peptide (aa1-23) replaced with TN…

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pSEVA6213S

Plasmid

#122094

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Publication

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Promoter

Pem7

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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hMEF2A K403R

Plasmid

#118355

Purpose

This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

Depositor

Lea Sistonen

Publication

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K403R

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-hHSF2 shRNA

Plasmid

#118346

Purpose

This plasmid expresses shRNA against hHSF2.

Depositor

Lea Sistonen

Publication

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF2 shRNA (HSF2 Human)

Expression

Mammalian

Mutation

WT

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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