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We narrowed to 16,242 results for: grna

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Showing: 2881 - 2900 of 16242 results

  6. pORANGE_NFL linker-3xFLAG KI

    Plasmid
    #182679
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182679-plasmid-map-sequence-id-363017

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  8. All_in_one_CRISPR/Cas9_LacZ

    Plasmid
    #74293
    Purpose
    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening
    Depositor
    Lynne Postovit
    Publication
    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LacZ-alpha
    Cas9
    mCherry
    Use
    CRISPR
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    Lac promoter, SV40, and T7 promoter
    Available since
    May 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74293-plasmid-map-sequence-id-140421

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  11. Px330-EGFP-LRRK2-CRISPR/Cas9

    Plasmid
    #180437
    Purpose
    Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells
    Depositor
    Frank Soldner
    Publication
    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA targeting LRRK2-G2019S (LRRK2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    180437-plasmid-map-sequence-id-355854

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  14. pSCL475

    Plasmid
    #219503
    Purpose
    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans
    Depositor
    Seth Shipman
    Publication
    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492
    Insert
    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    Expression
    Yeast
    Promoter
    GAL7
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219503-plasmid-map-sequence-id-435230

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  16. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65768-plasmid-map-sequence-id-112655

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  19. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid
    #91249
    Purpose
    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91249-plasmid-map-sequence-id-181276

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Showing: 2881 - 2900 of 16242 results