We narrowed to 15,991 results for: LEA

1. pET15b-E20A-PvuRts1I

   Plasmid

   #86897

   Purpose

   express E20A variant PvuRts1I in ER2566 strain

   Depositor

   Matthias Bochtler

   Article

   Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

   Insert

   Pvurts1I (pvuRts1I )
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   E20A

   Promoter

   T7

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b-D70A-PvuRts1I

   Plasmid

   #86890

   Purpose

   express D70A variant PvuRts1I in ER2566 strain

   Depositor

   Matthias Bochtler

   Article

   Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

   Insert

   PvuRts1I (pvuRts1I )
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   D70A

   Promoter

   T7

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMES140

   Plasmid

   #71849

   Purpose

   Complementation of NME1 gene disruption or mutation in yeast

   Depositor

   David Clayton, Mark Schmitt

   Article

   Schmitt et al Mol Cell Biol. 1993 Dec;13(12):7935-41.

   Insert

   NME1 (NME1 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   NME1

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pST50Trc4-HPCNDHFR

   Plasmid

   #63998

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   Protein C (HPC); TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST50Trc2-HPCNDHFR

   Plasmid

   #63962

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   Protein C (HPC); TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST50Trc3-HPCNDHFR

   Plasmid

   #63980

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   Protein C (HPC); TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST50Trc3-FLAGHISNDHFR

   Plasmid

   #63983

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis & FLAG; TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pST50Trc1-HPCNDHFR

   Plasmid

   #63944

   Purpose

   Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   Protein C (HPC); TEV cleavable

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDL128

   Plasmid

   #43912

   Depositor

   Stephen Elledge

   Article

   Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

   Insert

   DIF1 (DIF1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   SML domain mutated PPMLINQRT->AAAAAAAA, S59G, …

   Available Since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pDL127

    Plasmid

    #43911

    Depositor

    Stephen Elledge

    Article

    Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

    Insert

    DIF1 (DIF1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    HUG domain mutated VGMRIRQ->AAAAAAA, S59, Q66H

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

11. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid Kit

    #1000000156

    Purpose

    The MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.

    Depositor

    Scott Lenaghan

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

12. CHLOROMODAS Kit

    Plasmid Kit

    #1000000269

    Purpose

    Use the CHLOROMODAS modular cloning toolkit (MoClo) for engineering the Chlamydomonas reinhardtii chloroplast.

    Depositor

    Tobias Erb

    Available Since

    Nov. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

13. pBAC-lacZ

    Plasmid

    #13422

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Bacterial

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-CRISPR

    Plasmid

    #160047

    Purpose

    For cloning sgRNA to knockout a specific gene in piggybac plasmid

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-nCas9n

    Plasmid

    #47929

    Purpose

    expression of an optimized Cas9 for genome-editing in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    nls-zcas9-nls
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pegRNA2_tevopreQ1

    Plasmid

    #212039

    Purpose

    Bacterial expression of the pegRNA for genome editing in E. coli

    Depositor

    Quanjiang Ji

    Article

    Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

    Insert

    pegRNA
    

    Use

    CRISPR

    Promoter

    J23119

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-As-crRNA

    Plasmid

    #78956

    Purpose

    Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-TRE3G-dCas9-HaloTag

    Plasmid

    #169448

    Purpose

    expression of dCas9-HaloTag

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9-HaloTag
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+ GCamp6-3NLS

    Plasmid

    #165401

    Purpose

    Expresses a nuclear localized genetically encoded calcium reporter.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    Nuclear Localization Signal
    

    Use

    Avian, xenopus, zebrafish

    Tags

    GCamp6

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-U6-sgRNA-CMV-puro

    Plasmid

    #169450

    Purpose

    backbone for sgRNA expression

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    sgRNA
    

    Use

    Lentiviral

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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