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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,310 results for: LEA

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Showing: 2901 - 2920 of 12310 results

  3. pcDNA-GFP-EphrinB3-Flag

    Plasmid
    #155012
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1
    Depositor
    Sinisa Urban
    Publication
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EphrinB3 (EPHB3 Human)
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155012-plasmid-map-sequence-id-310419

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  6. SiC-V2-Cas9G7

    Plasmid
    #133043
    Purpose
    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.
    Depositor
    Sriram Neelamegham
    Publication
    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1
    Insert
    Tet repressor
    Use
    Lentiviral
    Tags
    Cerulean
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    133043-plasmid-map-sequence-id-264424

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  10. pOCC72

    Plasmid
    #118866
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric EGFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mEGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mEGFP, cleavabl…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118866-plasmid-map-sequence-id-232323

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  12. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  14. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid
    #98041
    Purpose
    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sa-dCas9-NLS-3xFLAG
    Use
    CRISPR
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    D10A + H557A
    Promoter
    CMV
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98041-plasmid-map-sequence-id-398173

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  15. pYFP-ErbB2

    Plasmid
    #66948
    Purpose
    Mammalian expression of rat ErbB2 tagged with YFP
    Depositor
    Mads Lerdrup, Bo van_Deurs
    Publication
    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ErbB2 (Erbb2 Rat)
    Tags
    6xHis, V5 Tag, and YFP
    Expression
    Mammalian
    Mutation
    (See depositor comment below on mutations A24T an…
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  16. pGL3Basic-ME.2/ApoEpromoter

    Plasmid
    #51436
    Purpose
    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer
    Depositor
    Sohail Tavazoie
    Publication
    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    APOE apolipoprotein E (APOE, ME2 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    The ME.2 enhancer is fused upstream of the ApoE g…
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51436-plasmid-map-sequence-id-177530

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  18. pET22b_EcSSB_2

    Plasmid
    #78204
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Publication
    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-
    Insert
    ssb (W88C)
    Expression
    Bacterial
    Mutation
    W88C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78204-plasmid-map-sequence-id-151737

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Showing: 2901 - 2920 of 12310 results