We narrowed to 3,815 results for: LYC;

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299_pETcon_SARS2_EG-5

Plasmid

#222232

Purpose

yeast surface display of the SARS-CoV-2 EG.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 EG.5 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Quartet-SpyTag

Plasmid

#214726

Purpose

In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with a C-terminal SpyTag fusion

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Quartet-SpyTag (S Synthetic)

Tags

SpyTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag-Quartet_NoLinker

Plasmid

#214731

Purpose

In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 with no linkers between the RBDs and an N-terminal SpyTag

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

SpyTag-Quartet_NoLinker (S Synthetic)

Tags

SpyTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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plenti hSyn AldoC

Plasmid

#220917

Purpose

Neuronal specific expression of AldoC

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

AldoC (ALDOC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hSyn

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI N-FLAG HIPK2

Plasmid

#213004

Purpose

expresses mouse HIPK2

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

HIPK2 (Hipk2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-RnCENP-O-GFP

Plasmid

#205179

Purpose

Expresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

Insert

CENP-O (Cenpo Rat)

Use

In vitro transcription

Tags

5 glycines linker followed by GFP

Expression

Mammalian

Mutation

Wild type rat CENP-O

Promoter

T7

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-HNRNPD_sgRNA2

Plasmid

#201599

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HNRNPD (HNRNPD Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PABPC1_sgRNA2

Plasmid

#201609

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PABPC1 (PABPC1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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PKC1-2GFP-ter-TRP1

Plasmid

#184773

Purpose

Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PKC1 (PKC1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRB131

Plasmid

#180174

Purpose

pAAV construct GluN1A714L (shRNA resistant)

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

AAV

Tags

Phluorin

Expression

Mammalian

Mutation

A714L

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-C.37-AVI

Plasmid

#181699

Purpose

Expresses SARS-CoV-2 RBD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-C.37 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L452Q, F490S

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:Y696G

Plasmid

#98156

Purpose

Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:Y696G

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tyrosine 696 to glycine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-TTBG

Plasmid

#115232

Purpose

Lentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)

Expression

Mammalian

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO FAPP2-GLTPH

Plasmid

#170739

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

Insert

PLEKHA8-GLTPH (PLEKHA8 Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v3/wtRBD

Plasmid

#169468

Purpose

Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

Plasmid

#169466

Purpose

Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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AlkB-D135G

Plasmid

#160434

Purpose

A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

Depositor

Tao Pan

Article

Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

Insert

E. coli AlkB

Tags

His-tag

Expression

Bacterial

Mutation

Changed Aspartic acid 135 to Glycine; deleted the…

Promoter

T7

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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D63_Ld_100680

Plasmid

#135876

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

flagellar glycoprotein-like protein

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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gh146

Plasmid

#106805

Purpose

expression of gRNA targeting FKTN

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FKTN (FKTN Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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gh111

Plasmid

#106778

Purpose

expression of gRNA targeting B3GALNT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALNT1 (B3GALNT1 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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