We narrowed to 19,663 results for: Met

Showing: 2901 - 2920 of 19663 results

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pDS257

Plasmid

#34971

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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PU6::klp-12_sgRNA

Plasmid

#46170

Purpose

klp-12 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

klp-12 targeting sgRNA (klp-12 Synthetic)

Use

CRISPR

Expression

Worm

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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PARP15-c021

Plasmid

#173102

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP15 (PARP15 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

G287-N470, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9

Plasmid

#78903

Purpose

Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-CAG-CyRFP1

Plasmid

#84357

Purpose

Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent)

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

CyRFP1

Use

AAV

Expression

Mammalian

Mutation

Double FLEXed

Promoter

CAG

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM156/157

Plasmid

#84083

Purpose

target miRNA156/157 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM156/157

Expression

Plant

Promoter

2x35S

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-H10-mCherry

Plasmid

#66531

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H10

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H10

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-ERP2-MG

Plasmid

#175501

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSLI-sandwich(0807600)

Plasmid

#85790

Purpose

P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R

Use

P. falciparum genomic integration

Tags

2xFKBP, GFP

Promoter

none

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT3/Cre

Plasmid

#89654

Purpose

Expresses a non-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

non-targeting gRNA

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSLI-N-sandwich-loxP(K13)

Plasmid

#85793

Purpose

P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

double 2xFKBP, GFP

Promoter

none

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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mEos4a-C1

Plasmid

#54810

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4a

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAC-4CL1

Plasmid

#35947

Depositor

Claudia Schmidt-dannert

Article

Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

Insert

4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLac-mod

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM160

Plasmid

#84085

Purpose

target miRNA160 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM160

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA_tdtomato_reporter1_SP-2x

Plasmid

#79370

Purpose

Assays activity of transcriptional activators fused to SP Cas9

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

tdtomato

Use

CRISPR

Expression

Mammalian

Available Since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA M3

Plasmid

#80936

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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UMG-SP-3_pET29b

Plasmid

#215435

Purpose

Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-3

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-tagBFP

Plasmid

#65257

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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modRNAc0-Cas9

Plasmid

#170180

Purpose

IVT template plasmid for making modified Cas9 RNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

Cas9

Use

Ivt template plasmid

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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