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pTAPmyc-T2A-tagRFP

Plasmid

#140278

Purpose

Backbone for making a mammalian expression vector of the protein of interest

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Empty backbone

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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epB_UbC_CymRV5-nls-GFP_DEx2

Plasmid

#119906

Purpose

ePiggyBac mammalian expression plasmid for CymR-GFP fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

CymRV5-nls-GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-AKAP-mtagBFP2-UBC6

Plasmid

#105007

Purpose

Mammalian Expression of mtagBFP2

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

mtagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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c-Rel cFlag pcDNA3

Plasmid

#20013

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

c-Rel (Rel Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
gRNA_AAVS1-T1

Plasmid

#41817

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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S-HBsAg

Plasmid

#103013

Purpose

Expresses HBV Small envelope glycoprotein in mammalian cells

Depositor

Wang-Shick Ryu

Article

Wang et al PLoS Pathog. 2010 Jul 15;6(7):e1000986.

Insert

HBV Small envelope protein

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCX-V5-H7N9-NS1

Plasmid

#99022

Purpose

Mammalian expression of NS1

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

NS1

Tags

V5

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pL7Ae-myc-T2A-tagRFP

Plasmid

#140263

Purpose

Expresses L7Ae in mammalian cells

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

L7Ae

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_minCMV

Plasmid

#62418

Purpose

MXS_chaining vector with minCMV

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Minimal CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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stathmin-GFP

Plasmid

#86782

Purpose

Mammalian expression of stathmin-GFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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px459 EQR

Plasmid

#101732

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Expression

Mammalian

Mutation

VQR

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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tTA_BFP

Plasmid

#58854

Purpose

Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

tTA transcription factor fused to enhanced blue fluorescent protein

Tags

BFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T2

Plasmid

#41820

Purpose

Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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epB_CAG_TetRFlag-nls-GFPx2_DEx2

Plasmid

#119910

Purpose

ePiggyBac mammalian expression plasmid for TetR-GFP fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

TetRFlag-nls-GFPx2

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S2

Plasmid

#128590

Purpose

Expresses tagless S2 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S2 (rpsB E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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GST-RNF126

Plasmid

#138643

Purpose

Bacterial expression of GST-RNF126 fusion protein

Depositor

Jane McGlade

Article

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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epB_UbC_CymRV5-nls-Halox2_DEx4

Plasmid

#119907

Purpose

ePiggyBac mammalian expression plasmid for CymR-Halo fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

CymRV5-nls-Halox2

Expression

Mammalian

Available Since

April 27, 2019

Availability

Academic Institutions and Nonprofits only

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miR155 in pcDNA3.1

Plasmid

#78126

Purpose

Mammalian expression of miR155

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

miR155 (MIR155 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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