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Plasmid#222113PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationACG start codonPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-UUG-IRES-mCherry
Plasmid#222112PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationUUG start codonPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUU-IRES-mCherry
Plasmid#222118PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationAUU start codonPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AGG-IRES-mCherry
Plasmid#222115PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationAGG start codonPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-AUC-IRES-mCherry
Plasmid#222117PurposeStart codon reporter with mutated start codonDepositorInsertGFP-IRES-mCherry
UseLentiviralTagsExpressionMutationAUC start codonPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5423_p3WJdB-glcO60
Plasmid#221194PurposeDNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor systemDepositorInsertT7 promoter-glcO60-3WJdB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoter with downstream glcO60 operatorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO-LPQTG-pET
Plasmid#221851PurposeExpresses SUMO-2 with sortase motif and His tagDepositorInsertSUMO-2 (SUMO2 Human)
UseTagsExpressionBacterialMutationGln88Leu, Gln89ProPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-Evectin-2
Plasmid#221754PurposeStable expression of Evectin-2 in mammalian cellsDepositorInsertPLEKHB2 (PLEKHB2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem PLEKHB2(PH domain) (amino acids 1-110)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PLCd1(PHD)
Plasmid#221753PurposeStable expression of GFP-PLCd1(PHD) in mammalian cellsDepositorInsertPLCd1 (PLCD1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–175PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-2xFYVE
Plasmid#221750PurposeStable expression of GFP-2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain
UseRetroviralTagsGFPExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-WIPI2b-mRuby3
Plasmid#221749PurposeStable expression of WIPI2b-mRuby3 in mammalian cellsDepositorInsertWIPI2b (WIPI2 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-STX17TM
Plasmid#221744PurposeStable expression of mRuby3-STX17TM in mammalian cellsDepositorInsertSTX17 (STX17 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationdeleted amino acids 1-228PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sik-YF
Plasmid#210723PurposeConstruct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (Ptk6 Mouse)
UseTagsExpressionMammalianMutationTyrosine 447 changed to PhenylalaninePromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121A wgMDH
Plasmid#204252PurposeBacterial expression of G121A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG121APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 I88A wgMDH
Plasmid#204249PurposeBacterial expression of I88A wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationI88APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Q58L wgMDH
Plasmid#204246PurposeBacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationQ58LPromoterT5Available sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196A wgMDH
Plasmid#204271PurposeBacterial expression of R196A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196E wgMDH
Plasmid#204272PurposeBacterial expression of R196E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196EPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123A wgMDH
Plasmid#204254PurposeBacterial expression of P123A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130Q wgMDH
Plasmid#204268PurposeBacterial expression of R130Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR130QPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only