162,050 results

1. CYBL-SPI1-CEBPB-IRF5

   Plasmid

   #190719

   Purpose

   For microglia differentiation from iPSC

   Depositor

   Li Gan

   Article

   Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

   Insert

   SPI1-CEBPB-IRF5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2lox-cAMPr

   Plasmid

   #99143

   Purpose

   Expresses cAMPr in mammalian cells

   Depositor

   Justin Blau

   Article

   Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

   Insert

   cAMPr
   

   Use

   Cre/Lox

   Tags

   cpGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_AncBE4max_P2A_GFP

   Plasmid

   #112100

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   AncBE4max_P2A_GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9CyMutL

   Plasmid

   #166981

   Purpose

   Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Inserts

   MutL (mutL )
   CymR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   E32K

   Promoter

   pCymRC and pLacI

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV/L7-6-GFP-WPRE

   Plasmid

   #126462

   Purpose

   An AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.

   Depositor

   Hirokazu Hirai

   Article

   Nitta et al Mol Ther Methods Clin Dev. 2017 Jul 27;6

   Insert

   L7-6, Purkinje cell specific promoter
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (AAV1)

   Viral Prep

   #100842-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (#100842). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   CAG

   Tags

   None

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

7. ABE8.8-m

   Plasmid

   #136294

   Purpose

   expresses ABE8.8-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.8-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC407 - pAAV EF1a eGFP

   Plasmid

   #60058

   Purpose

   An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   enhanced Green Fluorescent Protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAF0036 Ef1a-Si74-2a-EGFP

   Plasmid

   #193463

   Purpose

   Expression of Si74 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Si74
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3_cAMPFIRE-M

    Plasmid

    #182280

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiGuide_DKO

    Plasmid

    #183193

    Purpose

    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, mU6

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mitochondrial cpGFP

    Plasmid

    #215711

    Purpose

    Expresses cpGFP in the mitochondria

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and mitochondrial localization sequence…

    Expression

    Mammalian

    Mutation

    circularly permuted GFP

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPC-Flag-SOCS1

    Plasmid

    #129514

    Purpose

    Retroviral vector for the expression of human SOCS1 with a FLAG tag in N-terminal

    Depositor

    Gerardo Ferbeyre

    Article

    Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

    Insert

    SOCS1 (SOCS1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA_RFP-TEVprotease

    Plasmid

    #124947

    Purpose

    Expression of RFP-TEVprotease

    Depositor

    Matthew Good, Benjamin Schuster, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RFP-TEVprotease
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-SUMO1

    Plasmid

    #185637

    Purpose

    expression of GST-tagged SUMO1 in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

    Insert

    small ubiquitin-like modifier 1 (SUMO1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. LAMP1-RpHLuorin2

    Plasmid

    #171720

    Purpose

    Lysosomal expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-Sac-Abeta(M1-42)

    Plasmid

    #71875

    Purpose

    Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-42) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid

    #26730

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-GFP

    Plasmid

    #86537

    Purpose

    Retrovirus expressing GFP

    Depositor

    Xue Han

    Article

    Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin I (human)

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-Anillin

    Plasmid

    #68027

    Purpose

    Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

    Depositor

    Michael Glotzer

    Article

    Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

    Insert

    Anillin (ANLN Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    silent mutations at 798 5'-TGCC TCTTTGAATAAA…

    Promoter

    CMV

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGWB-cLUC

    Plasmid

    #174051

    Purpose

    Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

    Depositor

    Alberto Macho

    Article

    Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. AAV-FLEX-Arch-GFP (AAV1)

    Viral Prep

    #22222-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-FLEX-Arch-GFP (#22222). In addition to the viral particles, you will also receive purified AAV-FLEX-Arch-GFP plasmid DNA. CAG-driven cre-dependent Arch-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Promoter

    CAG

    Tags

    GFP (Cre-dependent)

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

23. pDonor MCS Rosa26

    Plasmid

    #37200

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Inserts

    Rosa26 left targeting arm
    MCS
    Rosa26 right targeting arm
    

    Use

    Genomic targeting - zinc finger

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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24. pZac2.1-GfaABC1D-Rpl22-HA

    Plasmid

    #111811

    Purpose

    Express Rpl22HA in astrocytes

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Ribosomal protein L22 (Rpl22 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBiFC-VC155

    Plasmid

    #22011

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VC155
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Alanine 206 to Lysine

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCAG-VSVL

    Plasmid

    #64085

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene

    Depositor

    Ian Wickersham

    Insert

    VSV polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

    Plasmid

    #58442

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCX-OKS-2A

    Plasmid

    #19771

    Purpose

    Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Science. 2008 Nov 7. 322(5903):949-53.

    Insert

    Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Three genes are connected with the foot-and-mouth…

    Promoter

    Available since

    Jan. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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29. hLRP1-pEZT-BM

    Plasmid

    #177985

    Purpose

    Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

    Depositor

    Lora Hooper

    Article

    Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

    Insert

    LDL receptor related protein 1 (LRP1 Human)
    

    Use

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAMPK alpha1 full length

    Plasmid

    #27297

    Depositor

    Morris Birnbaum

    Insert

    AMPK alpha1 (Prkaa1 Mouse)
    

    Use

    Tags

    his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge