We narrowed to 73,553 results for: LAS

1. pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

   Plasmid

   #20295

   Purpose

   Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Threonine

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSopBWT

   Plasmid

   #112512

   Purpose

   PsopB driven sopB and sigE

   Depositor

   Olivia Steele-Mortimer

   Article

   Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

   Insert

   sopB/sigE operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PsopB

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SH4-FRB-mRFP

   Plasmid

   #100741

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   Fyn
   

   Use

   Tags

   FRB and mRFP

   Expression

   Mammalian

   Mutation

   SH4 domain only

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA_GFP_mTLN1-FL-LBD

   Plasmid

   #164839

   Purpose

   Expression of GFP tagged FL mouse talin1 mutant

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

   Insert

   TLN1 (Tln1 Mouse)
   

   Use

   Tags

   His8-GFP

   Expression

   Mammalian

   Mutation

   Lipid binding deficient mutations

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-Xl-VSP1-GFP

   Plasmid

   #51883

   Purpose

   expression of Xl-VSP1 -GFP fusion in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r

   Plasmid

   #162958

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   triple FLAG epitope tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. POM42-mCitrine

   Plasmid

   #69854

   Purpose

   N-terminal tagging with mCitrine using URA3 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mCitrine minus start and stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYC44

   Plasmid

   #63903

   Purpose

   Initial Integrative Vector

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Use

   Tags

   FRT::3'UTRcta::NAT::FRT

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. C514-E44: attB5r-SNAP-nostop-attB1r

   Plasmid

   #162962

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   SNAP tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBL119-2

    Plasmid

    #104336

    Purpose

    Expresses thsR from a TetR regulated promoter under ATC control. Expresses sfGFP from a thsR regulated promoter. Expresses mCherry from a constituative promoter.

    Depositor

    Jeffrey Tabor

    Article

    Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

    Insert

    Thiosulfate response regulator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO1

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

    Plasmid

    #162967

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Dual Strep2 purification tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. POM43-mCitrine

    Plasmid

    #69855

    Purpose

    N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCitrine minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBB-80

    Plasmid

    #8814

    Depositor

    Cynthia Wolberger

    Article

    Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

    Insert

    GABPa1 (318-430) (Gabpa Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

14. C239-E03: attB2r-IRES-ffLuc2-pA-attB3

    Plasmid

    #162919

    Purpose

    Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    firefly luciferase with upstream IRES sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYC64

    Plasmid

    #63928

    Purpose

    Recipient replicative vector to construct FLAG tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYC62

    Plasmid

    #63927

    Purpose

    Recipient replicative vector to construct HA tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-NEO-CAG-BFP-pA-GFP-pA

    Plasmid

    #149344

    Purpose

    Vector for targeting the Replace reporter to the AAVS1 locus

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSuperRetro-puro-RalB 45i

    Plasmid

    #11122

    Depositor

    Christopher Counter

    Article

    Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

    Insert

    RalAB siRNA (RALA, RALB Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pFoxd3IRESGFPtv

    Plasmid

    #37266

    Depositor

    Patricia Labosky

    Article

    Hanna et al Genes Dev. 2002 Oct 15;16(20):2650-61.

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    Mouse Targeting

    Tags

    IRES-GFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pYC25

    Plasmid

    #63914

    Purpose

    Recipient overexpressing vector to construct YFP tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 (Saccharomyces cerevisiae)

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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