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pAAV-RSV-SpCas9

Plasmid

#85450

Purpose

Express SpCas9 in mammalian cells

Depositor

Hetian Lei

Article

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Has Service

AAV8

Insert

pRSV

Use

AAV

Promoter

pRSV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-dGBP1-TagBFP

Plasmid

#80086

Purpose

GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBRY-nuclear mCherry-IRES-PURO

Plasmid

#52409

Purpose

Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

nuclear mCherry

Expression

Mammalian

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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flk1 promoter

Plasmid

#26436

Depositor

Didier Stainier

Article

Jin et al Development. 2005 Dec . 132(23):5199-209

Insert

flk1 promoter

Use

Topo cloning vector

Available Since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pGIPz-mPD-L1

Plasmid

#121488

Purpose

Expression of mouse PD-L1 WT

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4 Bcl-2

Plasmid

#16461

Depositor

Bert Vogelstein

Article

Pietenpol et al Cancer Res. 1994 Jul 15. 54(14):3714-7.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pTrEno

Plasmid

#115474

Purpose

Eno-promoter driven expression vector for protein production in Trichoderma reesei

Depositor

Steve Decker

Article

Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d

Insert

Cel7A

Use

Unspecified

Mutation

Introns have been removed

Promoter

Trichoderma reesei Enolase (Eno) promoter

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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HPRT

Plasmid

#22884

Depositor

James Thomson

Article

Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Insert

hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)

Use

Cre/Lox; Homologous recombination

Mutation

PGK, NeoR, and BGH polyA are between the two geno…

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only

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pET-28c S2

Plasmid

#171920

Purpose

Express RNA-based (p)ppGpp sensor

Depositor

Mingxu You

Article

Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

Insert

RNA-based (p)ppGpp sensor

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUB_smHA_KDM5B_MS2

Plasmid

#81085

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smHA_KDM5B_24xMS2

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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V5-GFP-TurboID-KDEL_pLX208

Plasmid

#202020

Purpose

expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-TurboID-KDEL

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-dsRed-recipient

Plasmid

#11658

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling dsRed expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

dsRed

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-EGFP

Plasmid

#65264

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-mApg5

Plasmid

#22958

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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EF13E

Plasmid

#42942

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

EN- Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

AgeI-BclI fragment swapped with nt 1927-3708 from…

Promoter

CMV MIE, L1 5'UTR

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only
PB-gRNA-Puro

Plasmid

#121121

Purpose

Expression of a gRNA together with a Puromycin resistance

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

gRNA

Use

CRISPR

Expression

Mammalian

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-yellow pre-eGRASP(p32)

Plasmid

#111582

Purpose

An AAV vector that expresses yellow pre-eGRASP(p32) under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p32)

Use

AAV

Promoter

tetO

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTA-attB

Plasmid

#18937

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attB

Expression

Bacterial

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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pLVTH-siGFP

Plasmid

#12248

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

shRNA against GFP

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Mutation

NGFRdel is in place of GFP on the backbone.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-RT003-GPM

Plasmid

#163383

Purpose

To express sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

GFP-PDGFRtm-2A-mCherry (GPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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