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Showing: 561 - 600 of 2723 results

  2. pCMVtrunc LifeactΔC4-msfGFPΔN7

    Plasmid
    #231555
    Purpose
    Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Lifeact (ABP140 Budding Yeast)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMVtrunc
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231555-plasmid-map-sequence-id-459696

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  3. pCMVtrunc msfGFPΔC9-LifeactΔN2

    Plasmid
    #231556
    Purpose
    Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Lifeact (ABP140 Budding Yeast)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMVtrunc
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231556-plasmid-map-sequence-id-459689

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  8. PB CAG-F-bpA-2xSV40pA-F-td-sfGFP

    Plasmid
    #133572
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133572-plasmid-map-sequence-id-274057

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  9. PB CAG-L-bpA-3xSV40pA-L-td-sfGFP

    Plasmid
    #133577
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133577-plasmid-map-sequence-id-274079

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  10. PB CAG-F-bpA-3xSV40pA-F-td-sfGFP

    Plasmid
    #133573
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133573-plasmid-map-sequence-id-274063

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  11. PB CAG-L-bpA-2xSV40pA-L-td-sfGFP

    Plasmid
    #133576
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133576-plasmid-map-sequence-id-274071

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  12. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #133575
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133575-plasmid-map-sequence-id-274068

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  13. PB CAG-R-bpA-3xSV40pA-R-td-sfGFP

    Plasmid
    #133579
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133579-plasmid-map-sequence-id-274429

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  14. PB CAG-R-bpA-2xSV40pA-R-td-sfGFP

    Plasmid
    #133578
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133578-plasmid-map-sequence-id-274080

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  15. PB CAG-F-bpA-SV40pA-F-td-sfGFP

    Plasmid
    #133571
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133571-plasmid-map-sequence-id-274056

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  23. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215010
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215010-plasmid-map-sequence-id-429275

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  24. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215011
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215011-plasmid-map-sequence-id-429274

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  27. pHAGE pGK ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

    Plasmid
    #215013
    Purpose
    Express a ERphagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    KDEL
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215013-plasmid-map-sequence-id-429268

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  30. CUP1-∆ssCPY*-mGFP

    Plasmid
    #212197
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake, Chris MacDonald
    Insert
    Carboxypeptidase Y variant (PRC1 Budding Yeast)
    Use
    Tags
    mEGFP (monomeric version EGFP, A206K mutation)
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    CUP1
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    212197-plasmid-map-sequence-id-450955

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  31. AICSDP-45: TTN-mEGFP

    Plasmid
    #114412
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114412-plasmid-map-sequence-id-220885

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  38. pHBS1051 TDP43 RRM-GFP F-Y

    Plasmid
    #107802
    Purpose
    To test the effect of sequence on TDP43 droplet properties
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All phenylalanine to tyrosine in LLPS reporter
    Promoter
    Available since
    April 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    107802-plasmid-map-sequence-id-208177

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  39. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

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  40. pHBS1050 TDP43 RRM-GFP N-Q

    Plasmid
    #107803
    Purpose
    To test the effect of sequence on TDP43 droplet properties
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All asparagine to glutamine in LLPS reporter
    Promoter
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    107803-plasmid-map-sequence-id-207915

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Showing: 561 - 600 of 2723 results