We narrowed to 553 results for: SAM activators

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pCMV(MinDis)-iCytSnFR_PM

Plasmid

#177743

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCytSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn iCyt_BrEt_SnFR_ER

Plasmid

#177756

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn iCyt_BrEt_SnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCyt_F_SnFR_ER

Plasmid

#177754

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCyt_F_SnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iCyt_BrEt_SnFR_ER

Plasmid

#177746

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCyt_BrEt_SnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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_pCMV(MinDis)-iCyt_F_SnFR_ER

Plasmid

#177744

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

_pCMV(MinDis)-iCyt_F_SnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iCytSnFR_ER

Plasmid

#177742

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCytSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101103

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101115

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to LPAR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

LPAR1-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101113

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to CXCR4

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

CXCR4-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101111

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to AVPR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

AVPR2-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJPF0117

Plasmid

#130267

Purpose

Lentiviral vector for the co-expression of cytoplasmic bPAC and nuclear tagBFP

Depositor

Hana El-Samad

Article

Fonseca et al bioRxiv 2020, 747030

Insert

bPAC

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Promoter

hPGK

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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MBP-rPKM2 WT

Plasmid

#107158

Purpose

MBP-rPKM2 WT for production and purification of rat PKM2 WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL WT

Plasmid

#107156

Purpose

MBP-rPKL WT for production and purification of rat PKL WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL WT

Plasmid

#107159

Purpose

For mammalian expression of Flag-hPKL WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL WT (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL S113D

Plasmid

#107161

Purpose

For mammalian expression of Flag-hPKL S113D

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113D (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to aspartate

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL S113A

Plasmid

#107160

Purpose

For mammalian expression of Flag-hPKL S113A

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113A (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to alanine

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S12TAG

Plasmid

#107155

Purpose

MBP-rPKL construct for production and purification of rat PKL pS12

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 12 to TAG for phosphoserine insertion

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S113TAG

Plasmid

#107157

Purpose

MBP-rPKL S113TAG for production and purification of rat PKL pS113

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 113 changed to TAG for phosphoserine inser…

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pdCas9

Plasmid

#46569

Purpose

Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9
CRISPR array

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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