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Showing: 281 - 300 of 1125 results

  3. pCMV-enCas12a-A3A-Y130F

    Plasmid
    #193652
    Purpose
    Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    dLb-enCas12a-hA3A-Y130F, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193652-plasmid-map-sequence-id-422135

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  10. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid
    #160138
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160138-plasmid-map-sequence-id-333508

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  14. pCMV-enCas12a-A3A-N57G

    Plasmid
    #193651
    Purpose
    Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    dLb-enCas12a-hA3A-N57G, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193651-plasmid-map-sequence-id-422137

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Showing: 281 - 300 of 1125 results