We narrowed to 24,357 results for: nes

1. NES-EGFP-cPHx1

   Plasmid

   #116853

   Purpose

   PI(3,4)P2 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   PLEKHA1 (PLEKHA1 )
   

   Use

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   Amino acids 169.329

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVITRO1-TagRFP-NES

   Plasmid

   #184449

   Purpose

   Cytosolic TagRFP expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   TagRFP-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-JNKAR1-NES

   Plasmid

   #84632

   Purpose

   Unimolecular, FRET-based JNK activity reporter; cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   ECFP-FHA1-JNKsubDD-Citrine-NES
   

   Use

   Tags

   6xHis, Citrine, ECFP, Nuclear export signal (NES)…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Cerulean-WW-NES

   Plasmid

   #84629

   Purpose

   Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   Cerulean-WW-NES
   

   Use

   Tags

   6xHis, Cerulean, Nuclear export signal (NES), T7 …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-NES-ZapCY2

   Plasmid

   #59016

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

   Insert

   NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pVITRO1-iRFP702-NES

   Plasmid

   #184457

   Purpose

   Cytosolic iRFP702 expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   iRFP702-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. NES-EGFP-PH-BTKx1

   Plasmid

   #183651

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 2-170

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-NES-ZapCmR1.1

   Plasmid

   #59014

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSOMA-T679A-NES

   Plasmid

   #118940

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-NES-OptoJNKi

    Plasmid

    #89739

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFX-Pickles 2.34NES

    Plasmid

    #100028

    Purpose

    Improved FRET biosensor for the measurement of BCR-ABL activity.

    Depositor

    Yusuke Ohba

    Article

    Horiguchi et al Cell Struct Funct. 2017 Feb 2;42(1):15-2

    Insert

    Pickles-2.34NES
    

    Use

    Pfx vector is derived from pcmv vector.

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. NES-PAmCherry-cPHx2

    Plasmid

    #116860

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    PAmCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem dimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. myc-actin-NES-1

    Plasmid

    #60618

    Purpose

    Encodes MYC tagged beta-actin with a mutation in the NES

    Depositor

    Primal de Lanerolle

    Article

    Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    MYC

    Expression

    Bacterial and Mammalian

    Mutation

    I178A, L180A

    Promoter

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pSems meGFP-farnesyl

    Plasmid

    #222949

    Purpose

    Expression of C-terminal fragment of HRAS responsible for farnesylation tagged with GFP in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    C-terminal fragment of HRAS (170-189) (HRAS Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    C-terminal fragment of HRAS (170-189)

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAC3-N-GFP-NES

    Plasmid

    #222606

    Purpose

    Expression of MAC3-tagged GFP-NES (N-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 3' nuclear exclusion sequence (NES)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. MAC3-C-GFP-NES

    Plasmid

    #222603

    Purpose

    Expression of MAC3-tagged GFP-NES (C-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 5' nuclear exclusion sequence (NES)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. NES-mAzGreen-Cyclin D1 (HP)

    Plasmid

    #215070

    Purpose

    Expresses NES-mAzGreen-Cyclin D1 (HP) in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    NES-mAzGreen

    Expression

    Mammalian

    Mutation

    Cyclin D1(HP) denotes mutations M56A, V60A, and W…

    Promoter

    LTR

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. NES-mAzGreen-Cyclin D1

    Plasmid

    #215069

    Purpose

    Expresses NES-mAzGreen-Cyclin D1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    NES-mAzGreen

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. H_NES 41kDa (BFP)

    Plasmid

    #201393

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Insert

    HIV_NES-BFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Linked VLP NES

    Plasmid

    #182432

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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